EA.hy926人脐静脉细胞融合细胞

价 格:¥1800

期 货:现货(冻存管发2支)

保藏中心:BTCC

资源编号:BTCC-1050

产地:中国

套 餐:

T25/复苏细胞 无血清冻存液 专用完全培养基500ml 南美特级胎牛血清500ml

详细资料说明书下载质检报告(COA)

Cell line nameEA.hy 926
SynonymsEA. hy 926; EA hy 926; EA-hy926; EAhy 926; EAHY-926; EA.Hy926; EA.hy926; EAhy926; EaHy926; Eahy926
AccessionBTCC-1050
Resource Identification InitiativeTo cite this cell line use: EA.hy 926 (BTCC-1050)
CommentsGroup: Space-flown cell line (cellonaut).
Characteristics: Produced by the fusion of A549/8 with human umbilical vein endothelial cells.
Doubling time: 12 hours (PubMed=6407019).
Omics: Transcriptome analysis by microarray.
Anecdotal: Have been flown in space on Shijian-10 and on the ISS in 2016 to study growth and secretion in microgravity (PubMed=28199884; PubMed=30108515; PubMed=30977987).
Misspelling: EA.hy 296; Note=Occasionally.
Misspelling: EA-hy 296; Note=Occasionally.
Misspelling: EA.hy296; Note=Occasionally.
Sequence variations
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Species of originHomo sapiens (Human) (NCBI Taxonomy: 9606)
HierarchyParent: CVCL_IY87 (A549/8)
Sex of cellMale
CategoryHybrid cell line
STR profileSource(s): ATCC

Markers:
AmelogeninX
CSF1PO10,11,12
D5S81811
D7S8208,9,10
D13S31711
D16S53911,12
TH016,8,9.3
TPOX8,9
vWA14,17

Run an STR similarity search on this cell line
Publications

PubMed=6407019; DOI=10.1073/pnas.80.12.3734
Edgell C.-J.S., McDonald C.C., Graham J.B.
Permanent cell line expressing human factor VIII-related antigen established by hybridization.
Proc. Natl. Acad. Sci. U.S.A. 80:3734-3737(1983)

PubMed=3130908; DOI=10.1182/blood.V71.6.1669.1669
Emeis J.J., Edgell C.-J.S.
Fibrinolytic properties of a human endothelial hybrid cell line (Ea.hy 926).
Blood 71:1669-1675(1988)

PubMed=7791520; DOI=10.1016/0024-3205(95)00227-W
Ahn K., Pan S., Beningo K., Hupe D.
A permanent human cell line (EA.hy926) preserves the characteristics of endothelin converting enzyme from primary human umbilical vein endothelial cells.
Life Sci. 56:2331-2341(1995)

PubMed=12453433; DOI=10.1006/mvre.2002.2434
Unger R.E., Krump-Konvalinkova V., Peters K., Kirkpatrick C.J.
In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R.
Microvasc. Res. 64:384-397(2002)

PubMed=16575254; DOI=10.1097/01.mbc.0000220237.99843.a1
Boerma M., Burton G.R., Wang J.-R., Fink L.M., McGehee R.E. Jr., Hauer-Jensen M.
Comparative expression profiling in primary and immortalized endothelial cells: changes in gene expression in response to hydroxy methylglutaryl-coenzyme A reductase inhibition.
Blood Coagul. Fibrinolysis 17:173-180(2006)

PubMed=26345505; DOI=10.2144/000114326
Lieber D., Hochdorfer D., Stoehr D., Schubert A., Lotfi R., May T., Wirth D., Sinzger C.
A permanently growing human endothelial cell line supports productive infection with human cytomegalovirus under conditional cell growth arrest.
BioTechniques 59:127-136(2015)

PubMed=28199884; DOI=10.1016/j.biomaterials.2017.02.005
Pietsch J., Gass S., Nebuloni S., Echegoyen D., Riwaldt S., Baake C., Bauer J., Corydon T.J., Egli M., Infanger M., Grimm D.
Three-dimensional growth of human endothelial cells in an automated cell culture experiment container during the SpaceX CRS-8 ISS space mission -- the SPHEROIDS project.
Biomaterials 124:126-156(2017)

PubMed=30108515; DOI=10.3389/fphys.2018.01025
Li N., Wang C.-Z., Sun S.-J., Zhang C., Lu D.-Y., Chen Q., Long M.
Microgravity-induced alterations of inflammation-related mechanotransduction in endothelial cells on board SJ-10 satellite.
Front. Physiol. 9:1025.1-1025.15(2018)

PubMed=30977987; DOI=10.33594/000000071
Kruger M., Pietsch J., Bauer J., Kopp S., Carvalho D.T.O., Baatout S., Moreels M., Melnik D., Wehland M., Egli M., Sahana J., Kobbero S.D., Corydon T.J., Nebuloni S., Gass S., Evert M., Infanger M., Grimm D.
Growth of endothelial cells in space and in simulated microgravity -- a comparison on the secretory level.
Cell. Physiol. Biochem. 52:1039-1060(2019)

Cross-references
Cell line databases/resourcesCCRID; 1101HUM-PUMC000475
CCRID; 3101HUMGNHu39
Cell_Model_Passport; SIDM01986
Lonza; 685
Anatomy/cell type resourcesBTO; BTO:0000396
Biological sample resourcesBioSample; SAMN03471700
Cell line collections (Providers)ATCC; CRL-2922
BCRJ; 0345
CLS; 305034
KCB; KCB 2014048YJ
NCBI_Iran; C641
Chemistry resourcesChEMBL-Cells; CHEMBL4295452
ChEMBL-Targets; CHEMBL4296410
PubChem_Cell_line; CVCL_3901
Encyclopedic resourcesWikidata; Q54831722
Experimental variables resourcesEFO; EFO_0003039

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