Hacat人永生化角质形成细胞

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期 货:1周左右

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资源编号:BTCC-1056

产地:中国

套 餐:

细胞会员价 细胞非会员价 完全培养基500ml(仅供会员) 南美特级胎牛血清500ml(仅供会员)

详细资料说明书下载质检报告(COA)

Cell line nameHaCaT
SynonymsHaCAT; HACAT; Hacat
AccessionBTCC-1056
Resource Identification InitiativeTo cite this cell line use: HaCaT (BTCC-1056)
CommentsPopulation: Caucasian.
Virology: Susceptible to infection by Zika virus (ZIKV) (PubMed=29468137).
Doubling time: 28 hours (PubMed=32938951).
Omics: Deep antibody staining analysis.
Omics: Deep proteome analysis.
Omics: Metabolome analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Caution: Although HaCaT is distributed by a number of sources, the only official DKFZ-approved supplier is CLS.
Derived from site: In situ; Back, skin, epidermis; UBERON=UBERON_0001068+UBERON_0001003.
Cell type: Keratinocyte; CL=CL_0000312.
Sequence variations
  • Mutation; HGNC; 11998; TP53; Simple; p.His179Tyr (c.535C>T); ClinVar=VCV000127815; Zygosity=Heterozygous (PubMed=8504475).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg282Trp (c.843_844CC>TT); Zygosity=Heterozygous (PubMed=8504475).
HLA typingSource: CLS=300493
Class I
HLA-AA*31:01:02
HLA-BB*40:01:02,51:01:01
HLA-CC*03:04:01,15:02:01
Class II
HLA-DPDPB1*03:01:01,04:01:01
HLA-DQDQA1*01:02:01,03:03:01

DQB1*03:01:01,06:02:01
HLA-DRDRB1*04:01:01,15:01:01
Species of originHomo sapiens (Human) (NCBI Taxonomy: 9606)
HierarchyChildren:



CVCL_XK16 (HaCaT A-5)CVCL_HD65 (HaCaT BHLHE40 (-/-))CVCL_FA29 (HaCaT HSP70B'-GFP reporter)
CVCL_3868 (HaCaT II-4)CVCL_S024 (HaCaT SimpleCell O-GalNAc)CVCL_QX09 (HaCaT siYAP)
CVCL_QX10 (HaCaT YAP-5SA)CVCL_B0AY (HaCaT-16E5)CVCL_B0AZ (HaCaT-16E6)
CVCL_B0B0 (HaCaT-16E6E7)CVCL_B0B1 (HaCaT-16E7)CVCL_B0B2 (HaCaT-18E5)
CVCL_B0B3 (HaCaT-18E6)CVCL_B0B4 (HaCaT-18E7)CVCL_B0B5 (HaCaT-62E5)
CVCL_B0B6 (HaCaT-62E6)CVCL_B0B7 (HaCaT-62E7)CVCL_B0B8 (HaCaT-84E5)
CVCL_B0B9 (HaCaT-84E6)CVCL_B0BA (HaCaT-84E7)CVCL_B6YN (HaCaT-hGSTP1 ARE-SV40p-Luc2)
CVCL_B0BB (HaCaT-pLVX)CVCL_ZW69 (HaCaT-shNeg)CVCL_ZW70 (HaCaT-shNrf2#1)
CVCL_ZW71 (HaCaT-shNrf2#2)CVCL_ZW72 (HaCaT-shNrf2#3)CVCL_RL61 (HaCaT-TRS4)
CVCL_4Z60 (HaCaT/SM)CVCL_XK18 (HaCaT40degree)CVCL_BW57 (NOK-SI)
CVCL_A9W9 (nr-HaCat)

Sex of cellMale
Age at sampling62Y
CategorySpontaneously immortalized cell line
STR profileSource(s): CCRID; CLS; DSMZ; PubMed=11416159; PubMed=21868764; PubMed=35072517

Markers:
AmelogeninX
CSF1PO9,11
D1S165611,12
D2S44111
D2S133817,25
D3S135816
D5S81812
D6S104312,19
D7S8209,11
D8S117914
D10S124814,15
D12S39118,23
D13S31710,12
D16S5399,12
D18S5112
D19S43313,14
D21S1128 (DSMZ)
28,30.2 (CLS; PubMed=11416159; PubMed=35072517)
30.2 (CCRID)
D22S104515,16
FGA24 (CCRID; DSMZ; PubMed=11416159; PubMed=35072517)
24,27.2 (CLS)
Penta D11,13
Penta E7,12
SE3315,18.2
TH019.3
TPOX11,12
vWA16,17

Run an STR similarity search on this cell line
Web pageshttps://en.wikipedia.org/wiki/HaCaT
https://www.proteinatlas.org/learn/cellines
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-347.html
Publications

PubMed=2450098; DOI=10.1083/jcb.106.3.761
Boukamp P., Dzarlieva-Petrusevska R.T., Breitkreutz D., Hornung J., Markham A.F., Fusenig N.E.
Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
J. Cell Biol. 106:761-771(1988)

PubMed=8162337; DOI=10.1111/j.1600-0625.1993.tb00030.x
Schurer N., Kohne A., Schliep V., Barlag K., Goerz G.
Lipid composition and synthesis of HaCaT cells, an immortalized human keratinocyte line, in comparison with normal human adult keratinocytes.
Exp. Dermatol. 2:179-185(1993)

PubMed=8504475; DOI=10.1093/carcin/14.5.833
Lehman T.A., Modali R., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C.
p53 mutations in human immortalized epithelial cell lines.
Carcinogenesis 14:833-839(1993)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::aid-mc5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9406811; DOI=10.1111/1523-1747.ep12340708
Henseleit U., Zhang J., Wanner R., Haase I., Kolde G., Rosenbach T.
Role of p53 in UVB-induced apoptosis in human HaCaT keratinocytes.
J. Invest. Dermatol. 109:722-727(1997)

PubMed=10350023; DOI=10.1023/a:1011945212831
Altenburger R., Kissel T.
The human keratinocyte cell line HaCaT: an in vitro cell culture model for keratinocyte testosterone metabolism.
Pharm. Res. 16:766-771(1999)

PubMed=10523843; DOI=10.1038/sj.onc.1202934
Boukamp P., Popp S., Bleuel K., Tomakidi E., Burkle A., Fusenig N.E.
Tumorigenic conversion of immortal human skin keratinocytes (HaCaT) by elevated temperature.
Oncogene 18:5638-5645(1999)

PubMed=11583982; DOI=10.1016/S0002-9440(10)62541-2
Mueller M.M., Peter W., Mappes M., Huelsen A., Steinbauer H., Boukamp P., Vaccariello M.A., Garlick J.A., Fusenig N.E.
Tumor progression of skin carcinoma cells in vivo promoted by clonal selection, mutagenesis, and autocrine growth regulation by granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor.
Am. J. Pathol. 159:1567-1579(2001)

PubMed=15389883; DOI=10.1002/jcb.20212
Lemaitre G., Lamartine J., Pitaval A., Vaigot P., Garin J., Bouet S., Petat C., Soularue P., Gidrol X., Martin M.T., Waksman G.
Expression profiling of genes and proteins in HaCaT keratinocytes: proliferating versus differentiated state.
J. Cell. Biochem. 93:1048-1062(2004)

PubMed=20097197; DOI=10.1016/j.yexcr.2010.01.020
Rasanen K., Vaheri A.
Proliferation and motility of HaCaT keratinocyte derivatives is enhanced by fibroblast nemosis.
Exp. Cell Res. 316:1739-1747(2010)

PubMed=20100199; DOI=10.1111/j.1600-0625.2009.01026.x
Olaru F., Jensen L.E.
Chemokine expression by human keratinocyte cell lines after activation of Toll-like receptors.
Exp. Dermatol. 19:e314-e316(2010)

PubMed=21269460; DOI=10.1186/1752-0509-5-17
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Burckstummer T., Bennett K.L., Superti-Furga G., Colinge J.
Initial characterization of the human central proteome.
BMC Syst. Biol. 5:17.1-17.13(2011)

PubMed=21868764; DOI=10.1158/1078-0432.CCR-11-0690
Zhao M., Sano D., Pickering C.R., Jasser S.A., Henderson Y.C., Clayman G.L., Sturgis E.M., Ow T.J., Lotan R., Carey T.E., Sacks P.G., Grandis J.R., Sidransky D., Heldin N.-E., Myers J.N.
Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites.
Clin. Cancer Res. 17:7248-7264(2011)

PubMed=24155234; DOI=10.1007/7651_2013_42
Wilson V.G.
Growth and differentiation of HaCaT keratinocytes.
Methods Mol. Biol. 1195:33-41(2014)

PubMed=21185708; DOI=10.1016/j.jnutbio.2010.08.004
Serini S., Donato V., Piccioni E., Trombino S., Monego G., Toesca A., Innocenti I., Missori M., De Spirito M., Celleno L., Fasano E., Ranelletti F.O., Calviello G.
Docosahexaenoic acid reverts resistance to UV-induced apoptosis in human keratinocytes: involvement of COX-2 and HuR.
J. Nutr. Biochem. 22:874-885(2016)

PubMed=26989177; DOI=10.1242/dev.133728
Elbediwy A., Vincent-Mistiaen Z.I., Spencer-Dene B., Stone R.K., Boeing S., Wculek S.K., Cordero J., Tan E.H., Ridgway R., Brunton V.G., Sahai E., Gerhardt H., Behrens A., Malanchi I., Sansom O.J., Thompson B.J.
Integrin signalling regulates YAP and TAZ to control skin homeostasis.
Development 143:1674-1687(2016)

PubMed=29468137; DOI=10.5501/wjv.v7.i1.10
Himmelsbach K., Hildt E.
Identification of various cell culture models for the study of Zika virus.
World J. Virol. 7:10-20(2018)

PubMed=29660373; DOI=10.1016/j.bbagen.2018.04.012
Touat-Hamici Z., Bulteau A.-L., Bianga J., Jean-Jacques H., Szpunar J., Lobinski R., Chavatte L.
Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines.
Biochim. Biophys. Acta 1862:2493-2505(2018)

PubMed=31629070; DOI=10.1016/j.tiv.2019.104666
da Silva A.C.G., Chialchia A.R., de Castro E.G., e Silva M.R.L., Arantes D.A.C., Batista A.C., Kitten G.T., Valadares M.C.
A new corneal epithelial biomimetic 3D model for in vitro eye toxicity assessment: development, characterization and applicability.
Toxicol. In Vitro 62:104666.1-104666.7(2019)

PubMed=32938951; DOI=10.1038/s41598-020-71315-0
Pavez Lorie E., Stricker N., Plitta-Michalak B., Chen I.-P., Volkmer B., Greinert R., Jauch A., Boukamp P., Rapp A.
Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw.
Sci. Rep. 10:15196-15196(2020)

PubMed=33718544; DOI=10.1016/j.dib.2021.106871
Rusanov A.L., Romashin D.D., Zgoda V.G., Butkova T.V., Luzgina N.G.
Protein dataset of immortalized keratinocyte HaCaT cells and normal human keratinocytes.
Data Brief 35:106871.1-106871.6(2021)

PubMed=35072517; DOI=10.1289/EHP9676
Ferragut Cardoso A.P., Banerjee M., Al-Eryani L., Sayed M., Wilkey D.W., Merchant M.L., Park J.W., States J.C.
Temporal modulation of differential alternative splicing in HaCaT human keratinocyte cell line chronically exposed to arsenic for up to 28 wk.
Environ. Health Perspect. 130:17011.1-17011.16(2022)

PubMed=35647242; DOI=10.1016/j.dib.2022.108274
Romashin D.D., Rusanov A.L., Kozhin P.M., Karagyaur M.N., Tikhonova O.V., Zgoda V.G., Luzgina N.G.
Impact of p53 knockdown on protein dataset of HaCaT cells.
Data Brief 42:108274.1-108274.8(2022)

Cross-references
Cell line databases/resourcesCLDB; cl7229
CCRID; 1101HUM-PUMC000373
CCRID; 4201HUM-CCTCC00106
DSMZCellDive; ACC-771
Lonza; 56
TOKU-E; 3890
Anatomy/cell type resourcesBTO; BTO:0000552
Cell line collections (Providers)AddexBio; T0020001/117
BCRJ; 0341 - Discontinued
CCTCC; GDC0106
CLS; 300493
DSMZ; ACC-771 - Discontinued
ICLC; HL14001 - Discontinued
IZSLER; BS CL 168
KCB; KCB 200442YJ
Chemistry resourcesChEMBL-Cells; CHEMBL3308509
ChEMBL-Targets; CHEMBL614392
PubChem_Cell_line; CVCL_0038
Encyclopedic resourcesWikidata; Q5636366
Experimental variables resourcesEFO; EFO_0002056

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