15801533121

HCT-116人结直肠腺癌细胞

价 格：¥1800

期 货：现货（冻存管发2支）

保藏中心：BTCC

资源编号：BTCC-1060

产地：中国

套 餐：

T25/复苏细胞 无血清冻存液 专用完全培养基500ml 南美特级胎牛血清500ml

详细资料说明书下载质检报告（COA）

Cell line nameHCT 116
SynonymsHCT-116; HCT.116; HCT_116; HCT116; HCT116wt; CoCL2
AccessionCVCL_0291
Resource Identification InitiativeTo cite this cell line use: HCT 116 (RRID:CVCL_0291)
CommentsPart of: AstraZeneca Colorectal cell line (AZCL) panel.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: ENCODE project common cell types; tier 3.
Part of: FGFR genetic alteration cell panel (ATCC TCP-1034).
Part of: JFCR39 cancer cell line panel.
Part of: KuDOS 95 cell line panel.
Part of: MD Anderson Cell Lines Project.
Part of: NCI RAS program mutant KRAS cell line panel.
Part of: NCI-60 cancer cell line panel.
Population: Caucasian.
Doubling time: 17.1 hours (PubMed=22628656); 22 hours (PubMed=23546019); 25.02 hours (PubMed=25944804); 36 hours (PubMed=25984343); 18.14 +- 0.051 hours (PubMed=32927768); ~25-48 hours (DSMZ=ACC-581); 17.4 hours (NCI-DTP=HCT-116); ~21 hours (PBCF).
Microsatellite instability: Instable (MSI-high) (PubMed=9000147; PubMed=10674020; PubMed=12714694; PubMed=23671654; PubMed=24042735; PubMed=24755471; PubMed=25926053; PubMed=28683746; PubMed=31068700; Sanger).
Omics: Array-based CGH.
Omics: CTCF ChIP-seq epigenome analysis.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: CNV analysis.
Omics: Deep exome analysis.
Omics: Extracellular vesicles proteome analysis.
Omics: Deep phosphoproteome analysis.
Omics: Deep proteome analysis.
Omics: Deep quantitative phosphoproteome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: Fluorescence phenotype profiling.
Omics: HLA class I peptidome analysis by proteomics.
Omics: lncRNA expression profiling.
Omics: Metabolome analysis.
Omics: miRNA expression profiling.
Omics: N-glycan profiling.
Omics: Protein expression by reverse-phase protein arrays.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis by massively parallel signature sequencing (MPSS).
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Omics: Transcriptome analysis by serial analysis of gene expression (SAGE).
Misspelling: HCT-166; Note=In a BTO entry (BTO:0004743) now obsoleted.
Misspelling: HTC 116; Note=Occasionally.
Misspelling: HTC-116; Note=Occasionally.
Misspelling: Htc-116; Note=Occasionally.
Misspelling: HTC116; Note=Occasionally.
Misspelling: HTC1116; Note=Occasionally.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (PubMed=12615714; Cosmic-CLP; DepMap).
  • Mutation; HGNC; 1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (PubMed=17088437; Cosmic-CLP; DepMap).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (PubMed=17088437; DepMap).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Asp74fs*21 (c.220delG); Zygosity=Heterozygous (PubMed=17088437).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Glu33Argfs*20 (c.97delG); Zygosity=Heterozygous (DepMap).
  • Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (PubMed=9294210; PubMed=24755471; ATCC; Cosmic-CLP; DepMap).
  • Mutation; HGNC; 3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (Cosmic-CLP; DepMap).
  • Mutation; HGNC; 3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (PubMed=10700188; Cosmic-CLP; DepMap).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (PubMed=12068308; PubMed=17088437; PubMed=20570890; PubMed=24755471; PubMed=28683746; Cosmic-CLP; DepMap).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (PubMed=17088437; PubMed=20570890; PubMed=24755471; PubMed=28683746; Cosmic-CLP; DepMap).
  • Mutation; HGNC; 11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (PubMed=12615714).
  • Mutation; HGNC; 11998; TP53; None_reported; -; Zygosity=- (PubMed=9715273; PubMed=15900046; PubMed=16418264).
HLA typingSource: PubMed=15748285
Class I
HLA-AA*01,02
HLA-BB*18,45:01
HLA-CC*05,07
Class II
HLA-DPDPB1*03:01:01,04:02
HLA-DQDQB1*02,03

Source: PubMed=9178645
Class I
HLA-AA*01:01,02:01
HLA-BB*45:01
HLA-CC*05:01,07:01

Source: PubMed=25960936
Class I
HLA-AA*01:01,02:01
HLA-BB*18:01,45:01
HLA-CC*05:01,07:01
Class II
HLA-DQDQA1*05:02,05:02

DQB1*03:09,02:02
HLA-DRDRB1*03:05,11:02

Source: PubMed=26589293
Class I
HLA-AA*01:01,02:01
HLA-BB*18:01,45:01
HLA-CC*05:01,07:01

Source: CLS=300195
Class I
HLA-AA*01:01:01,02:01:01
HLA-BB*18:01:01,21:01:01
HLA-CC*05:01:01,07:01:01
Class II
HLA-DPDPB1*03:01:01G,04:02:01G
HLA-DQDQA1*05:01:01,05:05:01

DQB1*02:01:01,03:19:01
HLA-DRDRB1*03:01:01,11:02:01
Genome ancestrySource: PubMed=30894373

Origin% genome


African0
Native American0.08
East Asian, North1.16
East Asian, South0
South Asian0.97
European, North64.85
European, South32.93
DiseaseColon carcinoma (NCIt: C4910)
Species of originHomo sapiens (Human) (NCBI Taxonomy: 9606)
Originate from same individualCVCL_R729 ! HCT 116a
CVCL_R730 ! HCT 116b
Sex of cellMale
Age at sampling48Y
CategoryCancer cell line
STR profileSource(s): AddexBio; ATCC; CCRID; CLS; Cosmic-CLP; DSMZ; ECACC; KCLB; PubMed=11416159; PubMed=19372543; PubMed=25877200; PubMed=25926053

Markers:
AmelogeninX (CLS; PubMed=11416159; PubMed=19372543; PubMed=25877200; PubMed=25926053)
X,Y (AddexBio; ATCC; CCRID; Cosmic-CLP; DSMZ; ECACC; KCLB)
CSF1PO7,9,10,11 (Cosmic-CLP)
7,10 (AddexBio; ATCC; CCRID; CLS; DSMZ; ECACC; KCLB; PubMed=19372543; PubMed=25877200; PubMed=25926053)
D2S133816
D3S135812,18,19 (CCRID; CLS; DSMZ)
12,19 (PubMed=19372543; PubMed=25877200)
D5S81810,11
D7S82011,12
D8S117910,12,14,15 (CLS)
11,12,13,14 (DSMZ)
12,14 (CCRID; PubMed=19372543; PubMed=25877200)
D13S31710,12
D16S53911,12,13,14 (DSMZ)
11,13 (AddexBio; ATCC; CCRID; CLS; Cosmic-CLP; ECACC; PubMed=19372543; PubMed=25877200; PubMed=25926053)
D18S5116,17 (CCRID; CLS; DSMZ; PubMed=19372543)
17 (PubMed=25877200)
D19S43312,13
D21S1129,30
FGA18,23
Penta D9,13
Penta E12,13,14 (CLS)
13,14 (DSMZ; PubMed=25877200)
TH018,9 (AddexBio; ATCC; CCRID; CLS; Cosmic-CLP; DSMZ; ECACC; KCLB; PubMed=11416159; PubMed=19372543; PubMed=25877200)
8,9,10 (PubMed=25926053)
TPOX8 (ECACC; KCLB; PubMed=19372543; PubMed=25877200; PubMed=25926053)
8,9 (AddexBio; ATCC; CCRID; CLS; Cosmic-CLP; DSMZ)
vWA17 (PubMed=11416159; PubMed=19372543)
17,21,22,23 (DSMZ)
17,22 (AddexBio; ATCC; CCRID; CLS; Cosmic-CLP; ECACC; KCLB; PubMed=25877200; PubMed=25926053)

Run an STR similarity search on this cell line
Web pageshttps://en.wikipedia.org/wiki/HCT116_cells
https://dtp.cancer.gov/discovery_development/nci-60/cell_list.htm
https://www.synapse.org/#!Synapse:syn31544583
https://strap.nci.nih.gov/celline_detail.php?sample_id=17
https://www.cellosaurus.org/pawefish/ColonCellLineDescriptions/HCT116.html
http://genome.ucsc.edu/ENCODE/protocols/cell/human/HCT-116_protocol.pdf
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-88.html
https://tcpaportal.org/mclp/
Cell line databases/resourcesCLO; CLO_0003665
MCCL; MCC:0000181
CLDB; cl1574
CLDB; cl1575
CLDB; cl5198
CCRID; 1101HUM-PUMC000158
CCRID; 1101HUM-PUMC000331
CCRID; 3101HUMTCHu99
CCRID; 4201HUM-CCTCC00625
Cell_Model_Passport; SIDM00783
ColonAtlas; HCT116
Cosmic-CLP; 905936
DepMap; ACH-000971
DSMZCellDive; ACC-581
LINCS_LDP; LCL-1161
Lonza; 691
SKY/M-FISH/CGH; 1687
SKY/M-FISH/CGH; 2778
SKY/M-FISH/CGH; 2785
SKY/M-FISH/CGH; 3883
TOKU-E; 1422
Anatomy/cell type resourcesBTO; BTO:0001109
Biological sample resources4DN; 4DNSRMYUIVGD
BioSample; SAMN03470980
BioSample; SAMN03471481
BioSample; SAMN03473487
BioSample; SAMN05292433
BioSample; SAMN10988251
ENCODE; ENCBS004UZY
ENCODE; ENCBS009DQE
ENCODE; ENCBS257IHV
ENCODE; ENCBS378ENC
ENCODE; ENCBS389ENC
ENCODE; ENCBS409ENC
ENCODE; ENCBS409MTT
ENCODE; ENCBS431ENC
ENCODE; ENCBS471AAA
ENCODE; ENCBS472AAA
ENCODE; ENCBS475AAA
ENCODE; ENCBS476AAA
ENCODE; ENCBS494OPB
ENCODE; ENCBS615KTO
ENCODE; ENCBS626JHZ
ENCODE; ENCBS650OZU
ENCODE; ENCBS847SOB
ENCODE; ENCBS851PYB
Cell line collections (Providers)Abcam; ab255451
Abcam; ab273730
Abcam; ab288559
AddexBio; C0009005/41
ATCC; CCL-247
BCRC; 60349
BCRJ; 0288
CLS; 300195
DSMZ; ACC-581
ECACC; 91091005
ICLC; HTL95025
IZSLER; BS TCL 100
KCB; KCB 200706YJ
KCLB; 10247
NCBI_Iran; C570
NCI-DTP; HCT-116
RCB; RCB2979
Chemistry resourcesChEMBL-Cells; CHEMBL3308372
ChEMBL-Targets; CHEMBL394
GDSC; 905936
PharmacoDB; HCT116_517_2019
PubChem_Cell_line; CVCL_0291
Encyclopedic resourcesWikidata; Q28334584
Experimental variables resourcesEFO; EFO_0002824

更多>>相关产品

总部地址：北京市怀柔区渤海镇怀沙路536号

关注我们
Copyright © 2023 BTCC典型培养物保藏中心. All Rights Reserved. 京ICP备2023017635号-1
在线咨询
在线客服
电话咨询
公司电话：
15801533121
微信客服
返回顶部