HTR-8/SVneo 人绒毛膜滋养层细胞-BTCC典型培养物保藏中心-【BTCC细胞库】北京博沃尔斯生物科技有限公司官网

Cell line name

HTR-8/SVneo

Synonyms

HTR-8/SV neo; HTR-8/SV-neo; HTR8/SVneo; HTR8svn

Accession

BTCC-1076

Resource Identification Initiative

To cite this cell line use: HTR-8/SVneo (BTCC-1076)

Comments

Part of: ENCODE project common cell types; tier 3.
Characteristics: Useful to study trophoblast and placental biology (ATCC=CRL-3271).
Characteristics: Contains both epithelial and mesenchymal-like cell populations (PubMed=28161053).
Karyotypic information: Near triploid karyotype (PubMed=33096371).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [pSV3-neo].
Omics: DNA methylation analysis.
Derived from site: In situ; Trophoblast; UBERON=UBERON_0000088.

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_D728 (HTR-8)
Children:

CVCL_M689 (iC(3)-1)

Sex of cell

Sex unspecified

Age at sampling

6-12FW

Category

Transformed cell line

STR profile

Source(s): ATCC; PubMed=33810901

Markers:

Amelogenin	X
CSF1PO	12
D1S1656	16,17.3
D2S441	11
D2S1338	23,24
D3S1358	16
D5S818	12
D7S820	12
D8S1179	13,15
D10S1248	16
D12S391	19
D13S317	9,12
D16S539	13
D18S51	13
D19S433	14,15
D21S11	29,30
D22S1045	15
FGA	21,23
Penta D	9,12
Penta E	7,13
SE33	25.2,27.2
TH01	6,9.3
TPOX	8
vWA	13,18 (ATCC) 18 (PubMed=33810901)

Web pages

http://genome.ucsc.edu/ENCODE/protocols/cell/human/HTR8svn_Crawford_protocol.pdf
http://www.sonidel.com/sonidel/electroporation/nepa21-electroporator/cell-lines-htr-8-svneo-human-trophoblast-cells/

Publications

PubMed=7684692; DOI=10.1006/excr.1993.1139
Graham C.H., Hawley T.S., Hawley R.G., MacDougall J.R., Kerbel R.S., Khoo N., Lala P.K.
Establishment and characterization of first trimester human trophoblast cells with extended lifespan.
Exp. Cell Res. 206:204-211(1993)

PubMed=23325432; DOI=10.1101/gr.147942.112
Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M.
Dynamic DNA methylation across diverse human cell lines and tissues.
Genome Res. 23:555-567(2013)

PubMed=28161053; DOI=10.1016/j.placenta.2016.12.007
Abou-Kheir W., Barrak J., Hadadeh O., Daoud G.
HTR-8/SVneo cell line contains a mixed population of cells.
Placenta 50:1-7(2017)

PubMed=33096371; DOI=10.1016/j.placenta.2020.10.011
Weber M., Weise A., Vasheghani F., Gohner C., Fitzgerald J.S., Liehr T., Markert U.R.
Cytogenomics of six human trophoblastic cell lines.
Placenta 103:72-75(2021)

PubMed=33810901; DOI=10.1016/j.placenta.2021.02.022
Pastuschek J., Nonn O., Gutierrez-Samudio R.N., Murrieta-Coxca J.M., Muller J., Sanft J., Huppertz B., Markert U.R., Groten T., Morales-Prieto D.M.
Molecular characteristics of established trophoblast-derived cell lines.
Placenta 108:122-133(2021)

Cross-references

Cell line databases/resources

TOKU-E; 4099

Anatomy/cell type resources

BTO; BTO:0004125

Biological sample resources

ENCODE; ENCBS331AAA

Cell line collections (Providers)

ATCC; CRL-3271

Encyclopedic resources

Wikidata; Q54896677

Experimental variables resources

EFO; EFO_0005709

Gene expression databases

GEO; GSM816644

质检报告（COA）
Markers:

Amelogenin	X
CSF1PO	12
D1S1656	16,17.3
D2S441	11
D2S1338	23,24
D3S1358	16
D5S818	12
D7S820	12
D8S1179	13,15
D10S1248	16
D12S391	19
D13S317	9,12
D16S539	13
D18S51	13
D19S433	14,15
D21S11	29,30
D22S1045	15
FGA	21,23
Penta D	9,12
Penta E	7,13
SE33	25.2,27.2
TH01	6,9.3
TPOX	8
vWA	13,18 (ATCC) 18 (PubMed=33810901)

HTR-8/SVneo 人绒毛膜滋养层细胞

详细资料说明书下载质检报告（COA）

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