Ishikawa人子宫内膜癌细胞

价 格:¥1800

期 货:现货(冻存管发2支)

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资源编号:BTCC-1082

产地:中国

套 餐:

T25/复苏细胞 无血清冻存液 专用完全培养基500ml 南美特级胎牛血清500ml

详细资料说明书下载质检报告(COA)

Cell line nameIshikawa
SynonymsISHIKAWA; ISHI
AccessionCVCL_2529
Resource Identification InitiativeTo cite this cell line use: Ishikawa (RRID:CVCL_2529)
CommentsPart of: ENCODE project common cell types; tier 3.
Population: Japanese.
Doubling time: 36 hours (Note=At 9th passage), 29 hours (Note=At 40th passage), 27 hours (Note=At 50th passage) (DOI=10.1007/978-4-431-53981-0_2); 35.14 +- 2.68 hours (PubMed=23255909).
Omics: Array-based CGH.
Omics: Chromatin accessibility by ATAC-seq.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Misspelling: Ischikawaa; Note=Occasionally.
Derived from site: In situ; Endometrium; UBERON=UBERON_0001295.
Sequence variations
  • Mutation; HGNC; 8979; PIK3R1; Simple; p.Leu570Pro (c.1709T>C); Zygosity=Heterozygous (from child cell line Ishikawa (Heraklio) 02 ER-).
  • Mutation; HGNC; 9177; POLE; Simple; p.Pro102Ser (c.304C>T); Zygosity=Heterozygous (from child cell line Ishikawa (Heraklio) 02 ER-).
  • Mutation; HGNC; 9177; POLE; Simple; p.Asn751fs (c.2252delA); Zygosity=Heterozygous (from child cell line Ishikawa (Heraklio) 02 ER-).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Ser302fs*4 (c.906delC); Zygosity=Heterozygous (PubMed=20944090).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Glu288fs*3 (c.863delA); Zygosity=Heterozygous (from child cell line Ishikawa (Heraklio) 02 ER-).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Thr319fs*1 (c.950_953delTACT) (p.VL317fs) (V317fs*3); Zygosity=Heterozygous (PubMed=20944090).
  • Mutation; HGNC; 11998; TP53; Simple; p.Asp49His (c.145G>C); ClinVar=VCV000135948; Zygosity=Heterozygous (from child cell line Ishikawa (Heraklio) 02 ER-).
  • Mutation; HGNC; 11998; TP53; Simple; p.Met246Val (c.736A>G); ClinVar=VCV000100815; Zygosity=Heterozygous (PubMed=15901131).
DiseaseEndometrial adenocarcinoma (NCIt: C7359)
Species of originHomo sapiens (Human) (NCBI Taxonomy: 9606)
HierarchyChildren:



CVCL_W441 (EIIL)CVCL_N296 (IK-6)CVCL_9996 (IK-90)
CVCL_IR24 (ISH/DDP)CVCL_6543 (Ishikawa (Heraklio) 02 ER-)CVCL_8783 (Ishikawa (London) 02 ER+)
CVCL_W420 (Ishikawa 1-A-12)CVCL_W421 (Ishikawa 1-A-9)CVCL_W422 (Ishikawa 1-C-10)
CVCL_W423 (Ishikawa 1-D-9)CVCL_W424 (Ishikawa 1-E-8)CVCL_W425 (Ishikawa 1-F-6)
CVCL_W426 (Ishikawa 1-H-6)CVCL_W427 (Ishikawa 2-B-12)CVCL_W428 (Ishikawa 2-C-3)
CVCL_W429 (Ishikawa 2-C-7)CVCL_W430 (Ishikawa 2-E-7)CVCL_W431 (Ishikawa 2-F-4)
CVCL_W432 (Ishikawa 3-D-7)CVCL_W433 (Ishikawa 3-D-9)CVCL_D199 (Ishikawa 3-H-12)
CVCL_W434 (Ishikawa 3-H-3)CVCL_W435 (Ishikawa 3-H-4)CVCL_W436 (Ishikawa 3-H-7)
CVCL_IJ14 (Ishikawa-RP)

Sex of cellFemale
Age at sampling39Y
CategoryCancer cell line
STR profileSource(s): ECACC; PubMed=25877200

Markers:
AmelogeninX
CSF1PO11,12
D2S133820
D3S135815,17
D5S81810,11
D7S8209,10
D8S117912,16
D13S3179,12
D16S5399
D18S5113,19
D19S43312.2,14
D21S1128
FGA21
Penta D10,11
Penta E11,19
TH019,10
TPOX8
vWA14,17

Run an STR similarity search on this cell line
Web pageshttp://genome.ucsc.edu/ENCODE/protocols/cell/human/Ishikawa_Crawford_protocol.pdf
Publications

PubMed=4031568
Nishida M., Kasahara K., Kaneko M., Iwasaki H., Hayashi K.
Establishment of a new human endometrial adenocarcinoma cell line, Ishikawa cells, containing estrogen and progesterone receptors.
Nihon Sanka Fujinka Gakkai Zasshi 37:1103-1111(1985)

PubMed=10381137; DOI=10.1016/s0304-3835(98)00270-5
Ohta H., Sakamoto H., Satoh K.
In vitro effects of gonadotropin-releasing hormone (GnRH) analogue on cancer cell sensitivity to cis-platinum.
Cancer Lett. 134:111-118(1998)

PubMed=12703541; DOI=10.1111/j.1749-0774.2002.tb00105.x
Nishida M.
The Ishikawa cells from birth to the present.
Hum. Cell 15:104-117(2002)

DOI=10.1007/978-4-431-53981-0_2
Nishida M.
Ishikawa cells: opening of in vitro hormone research on endometrial carcinoma.
(In) Cell and molecular biology of endometrial carcinoma; Kuramoto H., Nishida M. (eds.); pp.35-58; Springer; Tokyo (2003)

PubMed=15053063
Kamata Y., Watanabe J., Hata H., Hamano M., Kuramoto H.
Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines.
Eur. J. Gynaecol. Oncol. 25:55-60(2004)

PubMed=15901131; DOI=10.1016/j.prp.2005.01.002
Murai Y., Hayashi S., Takahashi H., Tsuneyama K., Takano Y.
Correlation between DNA alterations and p53 and p16 protein expression in cancer cell lines.
Pathol. Res. Pract. 201:109-115(2005)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20218740; DOI=10.1177/153303461000900207
Wang Y.-M., Yang D., Cogdell D., Hu L.-M., Xue F.-X., Broaddus R., Zhang W.
Genomic characterization of gene copy-number aberrations in endometrial carcinoma cell lines derived from endometrioid-type endometrial adenocarcinoma.
Technol. Cancer Res. Treat. 9:179-189(2010)

PubMed=20944090; DOI=10.1126/scitranslmed.3001538
Dedes K.J., Wetterskog D., Mendes-Pereira A.M., Natrajan R., Lambros M.B., Geyer F.C., Vatcheva R., Savage K., Mackay A., Lord C.J., Ashworth A., Reis-Filho J.S.
PTEN deficiency in endometrioid endometrial adenocarcinomas predicts sensitivity to PARP inhibitors.
Sci. Transl. Med. 2:53ra75.1-53ra75.8(2010)

PubMed=22710073; DOI=10.1016/j.ygyno.2012.06.017
Korch C.T., Spillman M.A., Jackson T.A., Jacobsen B.M., Murphy S.K., Lessey B.A., Jordan V.C., Bradford A.P.
DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.
Gynecol. Oncol. 127:241-248(2012)

PubMed=23255909; DOI=10.3892/ol.2012.975
Zhao S.-Z., Li G.-X., Yang L., Li L., Li H.-Y.
Response-specific progestin resistance in a newly characterized Ishikawa human endometrial cancer subcell line resulting from long-term exposure to medroxyprogesterone acetate.
Oncol. Lett. 5:139-144(2013)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

Cross-references
Cell line databases/resourcesCCRID; 1101HUM-PUMC000686
Cell_Model_Passport; SIDM00038
LINCS_HMS; 50018
LINCS_LDP; LCL-1497
TOKU-E; 3966
Anatomy/cell type resourcesBTO; BTO:0003041
Biological sample resourcesBioSample; SAMN03471030
ENCODE; ENCBS228AAA
ENCODE; ENCBS229AAA
ENCODE; ENCBS230AAA
Cell line collections (Providers)BCRJ; 0364
ECACC; 99040201
Chemistry resourcesChEMBL-Cells; CHEMBL3307675
ChEMBL-Targets; CHEMBL614649
PharmacoDB; Ishikawa_667_2019
PubChem_Cell_line; CVCL_2529
Encyclopedic resourcesWikidata; Q54898281
Experimental variables resourcesEFO; EFO_0005718

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