ME-180人子宫颈表皮癌细胞

价 格:¥1800

期 货:现货(冻存管发2支)

保藏中心:BTCC

资源编号:BTCC-1104

产地:中国

套 餐:

T25/复苏细胞 无血清冻存液 专用完全培养基500ml 南美特级胎牛血清500ml

详细资料说明书下载质检报告(COA)

Cell line nameME-180
SynonymsMe-180; ME 180; ME180
AccessionBTCC-1104
Resource Identification InitiativeTo cite this cell line use: ME-180 (BTCC-1104)
CommentsPart of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Virology: Contains a complete HPV68 genome.
Doubling time: 1.5 days (PubMed=29156801).
Microsatellite instability: Stable (MSS) (Sanger).
Transformant: NCBI_TaxID; 45240; Human papillomavirus type 68 (HPV68).
Omics: Deep exome analysis.
Omics: Deep proteome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: Metastatic; Omentum; UBERON=UBERON_0003688.
Sequence variations
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (Cosmic-CLP; DepMap).
  • Mutation; HGNC; 11998; TP53; None_reported; -; Zygosity=- (PubMed=9887230).
HLA typingSource: PubMed=77569
Class I
HLA-AA*01,11
HLA-BB*05,08
HLA-CC*w07

Source: PubMed=26589293
Class I
HLA-AA*01:01,32:03
HLA-BB*08:01,44:02
HLA-CC*05:01,07:01
Genome ancestrySource: PubMed=30894373

Origin% genome


African1.51
Native American0.16
East Asian, North1.49
East Asian, South0
South Asian0
European, North64.3
European, South32.53
DiseaseHuman papillomavirus-related cervical squamous cell carcinoma (NCIt: C27676)
Squamous cell carcinoma of the cervix uteri (ORDO: Orphanet_213767)
Species of originHomo sapiens (Human) (NCBI Taxonomy: 9606)
HierarchyChildren:



CVCL_KS60 (CellSensor AP1-bla ME-180)CVCL_KS68 (CellSensor c-fos-bla ME-180)CVCL_KS52 (CellSensor GAS-bla ME-180)
CVCL_KS07 (CellSensor HRE-bla ME-180)CVCL_KB44 (CellSensor NFkB-bla ME-180)CVCL_KS28 (CellSensor SIE-bla ME-180)
CVCL_0473 (OV-2008)CVCL_6950 (SF767)CVCL_A342 (TSGH-8301)
Sex of cellFemale
Age at sampling66Y
CategoryCancer cell line
STR profileSource(s): ATCC; CCRID; CLS; Cosmic-CLP; JCRB; KCLB; PubMed=22710073; PubMed=25877200; PubMed=29156801; RCB; TKG

Markers:
AmelogeninX
CSF1PO11
D2S133818
D3S135816
D5S81812
D7S8209,10
D8S117914
D13S31711,12 (PubMed=22710073)
11,13 (ATCC; CCRID; CLS; Cosmic-CLP; JCRB; KCLB; PubMed=25877200; PubMed=29156801; RCB; TKG)
D16S53912,13
D18S5112
D19S43313,15.2
D21S1130,31
FGA23
Penta D9,14
Penta E12,14
TH018 (RCB)
8,9.3 (ATCC; CCRID; CLS; Cosmic-CLP; JCRB; KCLB; PubMed=22710073; PubMed=25877200; PubMed=29156801; TKG)
TPOX8,10
vWA15,16,17 (JCRB)
15,17 (ATCC; CCRID; CLS; Cosmic-CLP; KCLB; PubMed=22710073; PubMed=25877200; PubMed=29156801; RCB; TKG)

Run an STR similarity search on this cell line
Web pageshttps://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-612.html
Publications

PubMed=4317734; DOI=10.1093/jnci/45.1.107
Sykes J.A., Whitescarver J., Jernstrom P., Nolan J.F., Byatt P.
Some properties of a new epithelial cell line of human origin.
J. Natl. Cancer Inst. 45:107-122(1970)

DOI=10.1007/978-1-4757-1647-4_13
Biedler J.L.
Chromosome abnormalities in human tumor cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=77569; DOI=10.1111/j.1399-0039.1978.tb01259.x
Espmark J.A., Ahlqvist-Roth L., Sarne L., Persson A.
Tissue typing of cells in culture. III. HLA antigens of established human cell lines. Attempts at typing by the mixed hemadsorption technique.
Tissue Antigens 11:279-286(1978)

PubMed=7017212; DOI=10.1093/jnci/66.6.1003
Pollack M.S., Heagney S.D., Livingston P.O., Fogh J.
HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines.
J. Natl. Cancer Inst. 66:1003-1012(1981)

PubMed=7459858
Rousset M., Zweibaum A., Fogh J.
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)

PubMed=6220172
Dracopoli N.C., Fogh J.
Polymorphic enzyme analysis of cultured human tumor cell lines.
J. Natl. Cancer Inst. 70:469-476(1983)

PubMed=6500159; DOI=10.1159/000163283
Gershwin M.E., Lentz D., Owens R.B.
Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.
Exp. Cell Biol. 52:361-370(1984)

PubMed=6582512; DOI=10.1073/pnas.81.2.568
Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

PubMed=2990217
Yee C., Krishnan-Hewlett I., Baker C.C., Schlegel R., Howley P.M.
Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines.
Am. J. Pathol. 119:361-366(1985)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3371749; DOI=10.1016/0090-8258(88)90029-7
Grenman S.E., Shapira A., Carey T.E.
In vitro response of cervical cancer cell lines CaSki, HeLa, and ME-180 to the antiestrogen tamoxifen.
Gynecol. Oncol. 30:228-238(1988)

PubMed=8380785; DOI=10.1006/gyno.1993.1016
Iwasaka T., Oh-uchida M., Matsuo N., Yokoyama M., Fukuda K., Hara K., Fukuyama K., Hori K., Sugimori H.
Correlation between HPV positivity and state of the p53 gene in cervical carcinoma cell lines.
Gynecol. Oncol. 48:104-109(1993)

PubMed=7751322; DOI=10.1007/BF01366967
Rantanen V., Grenman S.E., Kulmala J., Grenman R.
The intrinsic radiosensitivity and sublethal damage repair capacity of five cervical carcinoma cell lines tested with the 96-well-plate assay.
J. Cancer Res. Clin. Oncol. 121:230-234(1995)

PubMed=8931502; DOI=10.3109/00016349609054706
Iwasaka T., Zheng P.-S., Ouchida M., Yamasaki H., Yokoyama M., Sugimori H.
Cytologic changes in two cervical carcinoma cell lines after transfection of the wild-type p53 gene.
Acta Obstet. Gynecol. Scand. 75:797-803(1996)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::aid-mc5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9887230; DOI=10.1006/gyno.1998.5194
Rantanen V., Grenman S.E., Kurvinen K., Hietanen S., Raitanen M., Syrjanen S.
p53 mutations and presence of HPV DNA do not correlate with radiosensitivity of gynecological cancer cell lines.
Gynecol. Oncol. 71:352-358(1998)

PubMed=15531914; DOI=10.1038/sj.onc.1208235
Baldus S.E., Schwarz E., Lohrey C., Zapatka M., Landsberg S., Hahn S.A., Schmidt D., Dienes H.-P., Schmiegel W.H., Schwarte-Waldhoff I.
Smad4 deficiency in cervical carcinoma cells.
Oncogene 24:810-819(2005)

PubMed=17325662; DOI=10.1038/sj.onc.1210330
Nakaya K., Yamagata H.D., Arita N., Nakashiro K.-I., Nose M., Miki T., Hamakawa H.
Identification of homozygous deletions of tumor suppressor gene FAT in oral cancer using CGH-array.
Oncogene 26:5300-5308(2007)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22710073; DOI=10.1016/j.ygyno.2012.06.017
Korch C.T., Spillman M.A., Jackson T.A., Jacobsen B.M., Murphy S.K., Lessey B.A., Jordan V.C., Bradford A.P.
DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.
Gynecol. Oncol. 127:241-248(2012)

PubMed=24134916; DOI=10.1186/1755-8166-6-44
McCormack A., Fan J.-L., Duesberg M., Bloomfield M., Fiala C., Duesberg P.H.
Individual karyotypes at the origins of cervical carcinomas.
Mol. Cytogenet. 6:44.1-44.23(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=27561551; DOI=10.1038/ncomms12645
Coscia F., Watters K.M., Curtis M., Eckert M.A., Chiang C.Y., Tyanova S., Montag A., Lastra R.R., Lengyel E., Mann M.
Integrative proteomic profiling of ovarian cancer cell lines reveals precursor cell associated proteins and functional status.
Nat. Commun. 7:12645.1-12645.14(2016)

PubMed=29156801; DOI=10.18632/oncotarget.21174
Kalu N.N., Mazumdar T., Peng S., Shen L., Sambandam V., Rao X., Xi Y., Li L., Qi Y., Gleber-Netto F.O., Patel A., Wang J., Frederick M.J., Myers J.N., Pickering C.R., Johnson F.M.
Genomic characterization of human papillomavirus-positive and -negative human squamous cell cancer cell lines.
Oncotarget 8:86369-86383(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line databases/resourcesCLO; CLO_0007657
CLO; CLO_0050924
CLDB; cl3429
CCRID; 3101HUMTCHu177
Cell_Model_Passport; SIDM00627
Cosmic-CLP; 687514
DepMap; ACH-001359
IGRhCellID; ME180%20GEO
LINCS_LDP; LCL-1305
TOKU-E; 2410
Biological sample resourcesBioSample; SAMN03471125
BioSample; SAMN03472007
BioSample; SAMN03472511
BioSample; SAMN03473047
Cell line collections (Providers)ATCC; HTB-33
ATCC; CRL-7932 - Discontinued
CLS; 300196
ICLC; HTL96027
JCRB; JCRB0810
KCLB; 30033
RCB; RCB2106
TKG; TKG 0437
Chemistry resourcesChEMBL-Cells; CHEMBL3308114
ChEMBL-Targets; CHEMBL1075499
GDSC; 687514
PharmacoDB; ME180_910_2019
PubChem_Cell_line; CVCL_1401
Encyclopedic resourcesWikidata; Q54904901
Experimental variables resourcesEFO; EFO_0006646

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