MRC-5人胚肺成纤维细胞(STR鉴定正确)

价 格:¥1800

期 货:现货(冻存管发2支)

保藏中心:BTCC

资源编号:BTCC-1114

产地:中国

套 餐:

T25/复苏细胞 无血清冻存液 专用完全培养基500ml 南美特级胎牛血清500ml

详细资料说明书下载质检报告(COA)

Cell line nameMRC-5
SynonymsMRC5; MRC 5; MRCV; MRC-V; Medical Research Council cell strain-5
AccessionBTCC-1114
Resource Identification InitiativeTo cite this cell line use: MRC-5 (BTCC-1114)
CommentsGroup: Vaccine production cell line.
Biotechnology: Used for the production of the Human Diploid Cell Vaccine (HDCV) against rabies (Trade name: Imovax Rabies).
Biotechnology: Used for the production of the Hepatitis A vaccines Avaxim, Epaxal, Havrix and Vaqta.
Biotechnology: Used for the production of the live varicella virus vaccine (Trade name: Varivax).
Biotechnology: Used for the production of the live zoster vaccine (Trade name: Zostavax).
Senescence: Capable of attaining 42-46 population doublings before the onset of senescence (ATCC=CCL-171). Senesces at 26 PDL (PubMed=17395773).
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Doubling time: 45 hours (Note=Lot 08072009), ~40 hours (Note=Lot 02272015) (JCRB=IFO50073); 36 hours (Note=Lot 041392), ~5 days (Note=Lot 031098), ~2 days (Note=Lot 05192003), ~1.5 days (Note=Lot 05202005), ~34 hours (Note=Lot 06022011), ~38 hours (Note=Lot 04212016) (JCRB=JCRB9008).
Omics: DNA methylation analysis.
Omics: Glycosphingolipids analysis.
Omics: Proteome analysis by 2D-DE.
Omics: Proteome analysis by 2D-DE/MS.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Misspelling: MCR-5; Note=Occasionally.
Derived from site: In situ; Fetal lung; UBERON=UBERON_0005597.
Cell type: Fibroblast of lung; CL=CL_0002553.
HLA typingSource: PubMed=31869419
Class I
HLA-AA*02:01,29:02
HLA-BB*07:02,44:02
HLA-CC*05:01,07:02
Species of originHomo sapiens (Human) (NCBI Taxonomy: 9606)
HierarchyChildren:



CVCL_2264 (1221)CVCL_2837 (A9(Neo1))CVCL_2838 (A9(Neo11))
CVCL_2844 (A9(Neo19))CVCL_2846 (A9(Neo20))CVCL_2848 (A9(Neo4))
CVCL_WT86 (AG27415)CVCL_F664 (Flow13000)CVCL_JG29 (HuS-L12/10)
CVCL_4W54 (HuSI-L23)CVCL_RG58 (iPSC D1)CVCL_RG59 (iPSC D4)
CVCL_RB14 (IRR-MRC-5)CVCL_4W64 (L12.28-1/1)CVCL_U651 (L12.9-2(1))
CVCL_U661 (L23immo)CVCL_E260 (MRC-5 CV1)CVCL_2621 (MRC-5 pd13)
CVCL_2622 (MRC-5 pd19)CVCL_2623 (MRC-5 pd25)CVCL_2624 (MRC-5 pd30)
CVCL_JF54 (MRC-5 PDL12)CVCL_H749 (MRC-5 PDL27)CVCL_H748 (MRC-5 PDL40)
CVCL_8B13 (MRC-5 PDL5.51)CVCL_2625 (MRC-5 SV1 TG1)CVCL_2626 (MRC-5 SV1 TG2)
CVCL_3027 (MRC-5-30)CVCL_3028 (MRC-5-40)CVCL_3029 (MRC-5-50)
CVCL_D690 (MRC-5V1)CVCL_2627 (MRC-5V2)CVCL_D923 (MRC-iPS-1)
CVCL_D924 (MRC-iPS-10)CVCL_D925 (MRC-iPS-100)CVCL_D926 (MRC-iPS-101)
CVCL_8343 (MRC-iPS-11)CVCL_D927 (MRC-iPS-12)CVCL_D928 (MRC-iPS-13)
CVCL_D929 (MRC-iPS-14)CVCL_D930 (MRC-iPS-15)CVCL_D931 (MRC-iPS-16)
CVCL_D932 (MRC-iPS-17)CVCL_8367 (MRC-iPS-18)CVCL_D933 (MRC-iPS-19)
CVCL_D934 (MRC-iPS-2)CVCL_D935 (MRC-iPS-20)CVCL_D936 (MRC-iPS-21)
CVCL_8426 (MRC-iPS-22)CVCL_D937 (MRC-iPS-23)CVCL_D938 (MRC-iPS-24)
CVCL_8433 (MRC-iPS-25)CVCL_D939 (MRC-iPS-26)CVCL_8434 (MRC-iPS-27)
CVCL_D940 (MRC-iPS-28)CVCL_D941 (MRC-iPS-29)CVCL_D942 (MRC-iPS-3)
CVCL_D943 (MRC-iPS-30)CVCL_D944 (MRC-iPS-31)CVCL_D945 (MRC-iPS-32)
CVCL_D946 (MRC-iPS-33)CVCL_D947 (MRC-iPS-34)CVCL_D948 (MRC-iPS-35)
CVCL_D949 (MRC-iPS-36)CVCL_D950 (MRC-iPS-37)CVCL_D951 (MRC-iPS-38)
CVCL_D952 (MRC-iPS-39)CVCL_D953 (MRC-iPS-4)CVCL_D954 (MRC-iPS-40)
CVCL_D955 (MRC-iPS-41)CVCL_D956 (MRC-iPS-42)CVCL_D957 (MRC-iPS-43)
CVCL_D958 (MRC-iPS-44)CVCL_D959 (MRC-iPS-45)CVCL_D960 (MRC-iPS-46)
CVCL_D961 (MRC-iPS-47)CVCL_D962 (MRC-iPS-48)CVCL_D963 (MRC-iPS-49)
CVCL_D964 (MRC-iPS-5)CVCL_D965 (MRC-iPS-50)CVCL_D966 (MRC-iPS-51)
CVCL_D967 (MRC-iPS-52)CVCL_D968 (MRC-iPS-53)CVCL_D969 (MRC-iPS-54)
CVCL_D970 (MRC-iPS-55)CVCL_D971 (MRC-iPS-56)CVCL_D972 (MRC-iPS-57)
CVCL_D973 (MRC-iPS-58)CVCL_D974 (MRC-iPS-59)CVCL_D975 (MRC-iPS-6)
CVCL_D976 (MRC-iPS-60)CVCL_D977 (MRC-iPS-61)CVCL_D978 (MRC-iPS-62)
CVCL_D979 (MRC-iPS-63)CVCL_D980 (MRC-iPS-64)CVCL_D981 (MRC-iPS-65)
CVCL_D982 (MRC-iPS-66)CVCL_D983 (MRC-iPS-67)CVCL_D984 (MRC-iPS-68)
CVCL_D985 (MRC-iPS-69)CVCL_D986 (MRC-iPS-7)CVCL_D987 (MRC-iPS-70)
CVCL_D988 (MRC-iPS-71)CVCL_D989 (MRC-iPS-72)CVCL_D990 (MRC-iPS-73)
CVCL_D991 (MRC-iPS-74)CVCL_D992 (MRC-iPS-75)CVCL_D993 (MRC-iPS-76)
CVCL_D994 (MRC-iPS-77)CVCL_D995 (MRC-iPS-78)CVCL_D996 (MRC-iPS-79)
CVCL_D997 (MRC-iPS-8)CVCL_D998 (MRC-iPS-80)CVCL_D999 (MRC-iPS-81)
CVCL_E000 (MRC-iPS-82)CVCL_E001 (MRC-iPS-83)CVCL_E002 (MRC-iPS-84)
CVCL_E003 (MRC-iPS-85)CVCL_E004 (MRC-iPS-86)CVCL_E005 (MRC-iPS-87)
CVCL_E006 (MRC-iPS-88)CVCL_E007 (MRC-iPS-89)CVCL_E008 (MRC-iPS-9)
CVCL_E009 (MRC-iPS-90)CVCL_E010 (MRC-iPS-91)CVCL_E011 (MRC-iPS-92)
CVCL_E012 (MRC-iPS-93)CVCL_E013 (MRC-iPS-94)CVCL_E014 (MRC-iPS-95)
CVCL_E015 (MRC-iPS-96)CVCL_E016 (MRC-iPS-97)CVCL_E017 (MRC-iPS-98)
CVCL_E018 (MRC-iPS-99)CVCL_C975 (MRC5-iPS12)CVCL_C974 (MRC5-iPS17)
CVCL_V234 (MRC5-iPS2)CVCL_C1XY (MRC5-iPS7)CVCL_C976 (MRC5-RiPS-1.11)
CVCL_C977 (MRC5-RiPS-1.2)CVCL_C978 (MRC5-RiPS-1.3)CVCL_C979 (MRC5-RiPS-1.8)
CVCL_C980 (MRC5-RiPS-1.9)CVCL_B0XS (MRC5/ACE2)CVCL_Y625 (SR2)
Sex of cellMale
Age at sampling14FW
CategoryFinite cell line
STR profileSource(s): AddexBio; ATCC; CCRID; JCRB; KCLB; PubMed=11416159; PubMed=21637780; PubMed=25877200; RCB

Markers:
AmelogeninX,Y
CSF1PO11,12
D2S133820
D3S135815,17
D5S81811,12
D7S82010,11
D8S117913
D13S31711,14
D16S5399,11
D18S5115,21
D19S43314,15
D21S1131.2
FGA21,23
Penta D12
Penta E12,16
TH018
TPOX8
vWA15

Run an STR similarity search on this cell line
Web pageshttps://en.wikipedia.org/wiki/MRC-5
https://www.atcc.org/en/support/technical-support/faqs/number-of-population-doublings-pdl-for-atcc-ccl-171
https://strap.nci.nih.gov/celline_detail.php?sample_id=68
https://micro.magnet.fsu.edu/primer/techniques/fluorescence/gallery/cells/mrc5/mrc5cells.html
https://www.olympus-lifescience.com/de/microscope-resource/galleries/confocal/fibroblastindex/
https://en.vircell.com/products/mrc-5-cell-line/
http://www.who.int/biologicals/BS%202077%20MRC-5.pdf
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-380.html
Publications

PubMed=4316953; DOI=10.1038/227168a0
Jacobs J.P., Jones C.M., Baille J.P.
Characteristics of a human diploid cell designated MRC-5.
Nature 227:168-170(1970)

PubMed=932048; DOI=10.1016/0092-1157(76)90018-4
Jacobs J.P.
The status of human diploid cell strain MRC-5 as an approved substrate for the production of viral vaccines.
J. Biol. Stand. 4:97-99(1976)

PubMed=1031681
Jacobs J.P.
Updated results on the karyology of the WI-38, MRC-5 and MRC-9 cell strains.
Dev. Biol. Stand. 37:155-156(1976)

PubMed=211143; DOI=10.1128/jcm.7.4.368-371.1978
Friedman H.M., Koropchak C.
Comparison of WI-38, MRC-5, and IMR-90 cell strains for isolation of viruses from clinical specimens.
J. Clin. Microbiol. 7:368-371(1978)

PubMed=113411; DOI=10.1016/S0092-1157(79)80043-8
Jacobs J.P., Garrett A.J., Merton R.
Characteristics of a serially propagated human diploid cell designated MRC-9.
J. Biol. Stand. 7:113-122(1979)

PubMed=6971834; DOI=10.1080/09553008114550251
Cooke A., Harris W.J.
Pyrimidine dimer excision in ageing human cells in tissue culture.
Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. 39:223-226(1981)

PubMed=7065527; DOI=10.1164/arrd.1982.125.2.222
Hay R.J., Williams C.D., Macy M.L., Lavappa K.S.
Cultured cell lines for research on pulmonary physiology available through the American Type Culture Collection.
Am. Rev. Respir. Dis. 125:222-232(1982)

PubMed=6538202; DOI=10.1083/jcb.98.3.1133
Azzarone B., Suarez H., Mingari M.C., Moretta L., Fauci A.S.
4F2 monoclonal antibody recognizes a surface antigen on spread human fibroblasts of embryonic but not of adult origin.
J. Cell Biol. 98:1133-1137(1984)

PubMed=3335022
Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski M.J., Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.
Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.
Cancer Res. 48:589-601(1988)

PubMed=1965304; DOI=10.1002/elps.1150111203
Celis J.E., Dejgaard K., Madsen P., Leffers H., Gesser B., Honore B., Rasmussen H.H., Olsen E., Lauridsen J.B., Ratz G., Mouritzen S., Basse B., Hellerup M., Celis A., Puype M., Van Damme J., Vandekerckhove J.
The MRC-5 human embryonal lung fibroblast two-dimensional gel cellular protein database: quantitative identification of polypeptides whose relative abundance differs between quiescent, proliferating and SV40 transformed cells.
Electrophoresis 11:1072-1113(1990)

PubMed=10752125
Polianskaia G.G., Efremova T.N., Sakuta G.A.
The effect of mycoplasmal contamination of human embryonic lung cell line MRC-5 on the karyotypic variability.
Tsitologiia 42:190-195(2000)

PubMed=17395773; DOI=10.1634/stemcells.2006-0504
Sudo K., Kanno M., Miharada K., Ogawa S., Hiroyama T., Saijo K., Nakamura Y.
Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.
Stem Cells 25:1610-1617(2007)

PubMed=19657000
Rubporn A., Srisomsap C., Subhasitanont P., Chokchaichamnankit D., Chiablaem K., Svasti J., Sangvanich P.
Comparative proteomic analysis of lung cancer cell line and lung fibroblast cell line.
Cancer Genomics Proteomics 6:229-237(2009)

PubMed=21637780; DOI=10.1371/journal.pgen.1002085
Nishino K., Toyoda M., Yamazaki-Inoue M., Fukawatase Y., Chikazawa E., Sakaguchi H., Akutsu H., Umezawa A.
DNA methylation dynamics in human induced pluripotent stem cells over time.
PLoS Genet. 7:E1002085-E1002085(2011)

PubMed=25903999; DOI=10.1002/biot.201400388
Genzel Y.
Designing cell lines for viral vaccine production: where do we stand?
Biotechnol. J. 10:728-740(2015)

PubMed=26477663; DOI=10.1038/srep14988
Ojima T., Shibata E., Saito S., Toyoda M., Nakajima H., Yamazaki-Inoue M., Miyagawa Y., Kiyokawa N., Fujimoto J.-I., Sato T., Umezawa A.
Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells.
Sci. Rep. 5:14988-14988(2015)

CLPUB00391
Wadman M.
The vaccine race: science, politics, and the human costs of defeating disease.
(In) ISBN 9780525427537; pp.1-436; Viking; New York (2016)

PubMed=31869419; DOI=10.1084/jem.20191164
Lubke M., Spalt S., Kowalewski D.J., Zimmermann C., Bauersfeld L., Nelde A., Bichmann L., Marcu A., Peper J.K., Kohlbacher O., Walz J.S., Le-Trilling V.T.K., Hengel H., Rammensee H.G., Stevanovic S., Halenius A.
Identification of HCMV-derived T cell epitopes in seropositive individuals through viral deletion models.
J. Exp. Med. 217:20191164.1-20191164.14(2020)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cross-references
Cell line databases/resourcesCLO; CLO_0007865
CLO; CLO_0050055
MCCL; MCC:0000336
CLDB; cl3563
CLDB; cl3564
CLDB; cl3566
CLDB; cl3567
CLDB; cl3568
CLDB; cl5168
CCRID; 1101HUM-PUMC000044
CCRID; 1102HUM-NIFDC00002
CCRID; 3101HUMGNHu41
CCRID; 3101HUMSCSP5040
CCRID; 4201HUM-CCTCC00032
CCRID; 5301HUM-KCB89023YJ
FCS-free; 179-2-338-1-16-3
Lonza; 52
Anatomy/cell type resourcesBTO; BTO:0001590
Biological sample resourcesBioSample; SAMN01821580
BioSample; SAMN01821702
BioSample; SAMN01821735
BioSample; SAMN03473183
Cell line collections (Providers)AddexBio; T0016002/4922
ATCC; CCL-171
BCRC; 60023
BCRJ; 0180
CCTCC; GDC0032
CLS; 300395 - Discontinued
ECACC; 97112601 - Discontinued
IBRC; C10313
ICLC; HL95001
IZSLER; BS CL 68
JCRB; IFO50073
JCRB; JCRB9008
KCB; KCB 89023YJ
KCB; KCB 200412YJ
KCLB; 10171
NCBI_Iran; C125
RCB; RCB0211
RCB; RCB0218
Chemistry resourcesChEMBL-Cells; CHEMBL3308499
ChEMBL-Targets; CHEMBL614181
PubChem_Cell_line; CVCL_0440
Encyclopedic resourcesWikidata; Q3273166
Experimental variables resourcesEFO; EFO_0002835
Gene expression databasesGEO; GSM248209
GEO; GSM248210
GEO; GSM520665
GEO; GSM520666
GEO; GSM520667
GEO; GSM579888
GEO; GSM579889
GEO; GSM607969
GEO; GSM3738752
GEO; GSM3738753
Proteomic databasesPRIDE; PXD001909
PRIDE; PXD013120
PRIDE; PXD023286

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