SK-GT-4人食道癌肿瘤细胞(STR鉴定正确)

价 格:¥2400

期 货:现货(冻存管发2支)

保藏中心:BTCC

资源编号:BTCC-1419

产地:中国

套 餐:

T25/复苏细胞 无血清冻存液 专用完全培养基500ml 南美特级胎牛血清500ml

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Cell line nameSK-GT-4
SynonymsSKGT4; SK4
AccessionBTCC-1419
Resource Identification InitiativeTo cite this cell line use: SK-GT-4 
CommentsPart of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
From: Memorial Sloan Kettering Cancer Center; New York; USA.
Population: Caucasian.
Doubling time: 39 hours (PubMed=8334620); ~30-35 hours (DSMZ=ACC-712).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Genomics; Whole genome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Caution: TP53 mutation indicated incorrectly as being at p.Arg175His (c.524G>A) in PubMed=7665247.
Misspelling: SJGT4; GEO=GSM337727.
Derived from site: In situ; Esophagus, distal; UBERON=UBERON_0013473.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ala (c.35G>C); ClinVar=VCV000045122; Zygosity=Heterozygous (PubMed=27594985; Cosmic-CLP=1503365; DepMap=ACH-001654).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu542Lys (c.1624G>A); ClinVar=VCV000031944; Zygosity=Heterozygous (Cosmic-CLP=1503365; DepMap=ACH-001654).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Gln100Ter (c.298C>T); ClinVar=VCV000634707; Zygosity=Homozygous (PubMed=20075370; Cosmic-CLP=1503365; DepMap=ACH-001654).
Genome ancestrySource: PubMed=30894373

Origin% genome


African2.11
Native American1.08
East Asian, North1.62
East Asian, South0
South Asian7.99
European, North27.72
European, South59.48
DiseaseEsophageal adenocarcinoma (NCIt: C4025)
Adenocarcinoma of the esophagus (ORDO: Orphanet_99976)
Species of originHomo sapiens (Human) (NCBI Taxonomy: 9606)
HierarchyChildren:

CVCL_C0X0 (SK4-Rf)
Sex of cellMale
Age at sampling89Y
CategoryCancer cell line
STR profileSource(s): Cosmic-CLP=1503365; DepMap=ACH-001654; DSMZ=ACC-712; PubMed=20075370

Markers:
AmelogeninX
CSF1PO11,15
D2S133816,19
D3S135817
D5S81812
D7S8207,11
D8S117913
D13S3179,10
D16S53911,12,13
D18S5114
D19S43313,14
D21S1131.2
FGA22,23
Penta D9
Penta E7
TH016,9.3
TPOX8,10
vWA17,19

Run an STR similarity search on this cell line
Publications

PubMed=8334620; DOI=10.1002/1097-0142(19930801)72:3<649::aid-cncr2820720305>3.0.CO;2-L
Altorki N.K., Schwartz G.K., Blundell M.L., Davis B.M., Kelsen D.P., Albino A.P.
Characterization of cell lines established from human gastric-esophageal adenocarcinomas. Biologic phenotype and invasion potential.
Cancer 72:649-657(1993)

PubMed=7665247; DOI=10.1002/ijc.2910640109
Nabeya Y., Loganzo F. Jr., Maslak P., Lai L., de Oliveira A.R., Schwartz G.K., Blundell M.L., Altorki N.K., Kelsen D.P., Albino A.P.
The mutational status of p53 protein in gastric and esophageal adenocarcinoma cell lines predicts sensitivity to chemotherapeutic agents.
Int. J. Cancer 64:37-46(1995)

PubMed=11520067; DOI=10.1006/bbrc.2001.5400
Kan T., Shimada Y., Sato F., Maeda M., Kawabe A., Kaganoi J.-i., Itami A., Yamasaki S., Imamura M.
Gene expression profiling in human esophageal cancers using cDNA microarray.
Biochem. Biophys. Res. Commun. 286:792-801(2001)

PubMed=20075370; DOI=10.1093/jnci/djp499; PMCID=PMC2902814
Boonstra J.J., van Marion R., Beer D.G., Lin L., Chaves P., Ribeiro C., Pereira A.D., Roque L., Darnton S.J., Altorki N.K., Schrump D.S., Klimstra D.S., Tang L.H., Eshleman J.R., Alvarez H., Shimada Y., van Dekken H., Tilanus H.W., Dinjens W.N.M.
Verification and unmasking of widely used human esophageal adenocarcinoma cell lines.
J. Natl. Cancer Inst. 102:271-274(2010)

PubMed=20139130; DOI=10.1093/carcin/bgq036
Duggan S.P., Behan F.M., Kirca M., Smith S.M., Reynolds J.V., Long A., Kelleher D.P.
An integrative genomic approach in oesophageal cells identifies TRB3 as a bile acid responsive gene, downregulated in Barrett's oesophagus, which regulates NF-kappaB activation and cytokine levels.
Carcinogenesis 31:936-945(2010)

PubMed=23795680; DOI=10.1111/dote.12095
Boonstra J.J., Tilanus H.W., Dinjens W.N.M.
Translational research on esophageal adenocarcinoma: from cell line to clinic.
Dis. Esophagus 28:90-96(2015)

PubMed=26317542; DOI=10.18632/oncotarget.4540; PMCID=PMC4694873
Chen Q.-R., Song S.-M., Wei S.-Z., Liu B., Honjo S., Scott A.W., Jin J.-K., Ma L., Zhu H.-T., Skinner H.D., Johnson R.L., Ajani J.A.
ABT-263 induces apoptosis and synergizes with chemotherapy by targeting stemness pathways in esophageal cancer.
Oncotarget 6:25883-25896(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=27594985; DOI=10.12688/f1000research.7033.1; PMCID=PMC4991527
Contino G., Eldridge M.D., Secrier M., Bower L., Elliott R.F., Weaver J., Lynch A.G., Edwards P.A.W., Fitzgerald R.C.
Whole-genome sequencing of nine esophageal adenocarcinoma cell lines.
F1000Research 5:1336.1-1336.12(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers)DSMZ; ACC-712
ECACC; 11012007
Cell line databases/resourcescancercelllines; CVCL_2195
Cell_Model_Passport; SIDM00483
Cosmic-CLP; 1503365
DepMap; ACH-001654
DSMZCellDive; ACC-712
Biological sample resourcesBioSample; SAMN03473395
CRISP screens repositoriesBioGRID_ORCS_Cell_line; 1011
Chemistry resourcesGDSC; 1503365
PharmacoDB; SKGT4_1389_2019
Encyclopedic resourcesWikidata; Q54953662
Experimental variables resourcesEFO; EFO_0001237
Gene expression databasesArrayExpress; E-MTAB-3610
GEO; GSE2144
GEO; GSE13376
GEO; GSM1374878
GEO; GSM1670431
Polymorphism and mutation databasesCosmic; 1599346
IARC_TP53; 4079
Proteomic databasesPRIDE; PXD030304
Sequence databasesEGA; EGAS00001000978

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