Cell line name | SH-SY5Y | ||||||||||||||||||||||||||||||||||||||||||||||||
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Synonyms | SH-Sy5y; SHSY5Y; SHSY-5Y; SK-SH-SY5Y; SY5Y; SH-SY5Y Parental | ||||||||||||||||||||||||||||||||||||||||||||||||
Accession | BTCC-1158 | ||||||||||||||||||||||||||||||||||||||||||||||||
Resource Identification Initiative | To cite this cell line use: SH-SY5Y (BTCC-1158) | ||||||||||||||||||||||||||||||||||||||||||||||||
Comments | Problematic cell line: Partially contaminated. Some laboratories that are redistributing this cell line are in fact redistributing a contaminated cell line of mouse origin (PubMed=25182563). Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE). Part of: ENCODE project common cell types; tier 3. From: Memorial Sloan Kettering Cancer Center; New York; USA. Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK 810. Population: Caucasian. Characteristics: Neuroblastic type (N-type) (PubMed=15720811). Characteristics: There seem to be differences in the retinoic acid (RA)-induced neuronal phenotype of SH-SY5Y cells from ATCC and ECACC. After 5 days of RA treatment, ECACC cells are slightly larger in size and contains significant amount of neuroblastic (N-type) cells and a small fraction of epithelial (S-type) cells (PubMed=18957096). Virology: Low susceptibility to infection by Zika virus (ZIKV) (PubMed=29468137). Doubling time: >55 hours (DSMZ=ACC-209). Omics: Deep antibody staining analysis. Omics: Deep exome analysis. Omics: Deep proteome analysis. Omics: Deep tyrosine phosphoproteome analysis. Omics: GPI-anchored proteins analysis by proteomics. Omics: Mitochondrial proteome analysis by 2D-DE/MS. Omics: N-glycan profiling. Omics: SNP array analysis. Omics: Transcriptome analysis by microarray. Omics: Transcriptome analysis by RNAseq. Misspelling: SH-SY5; Note=Occasionally. Derived from site: Metastatic; Bone marrow; UBERON=UBERON_0002371. | ||||||||||||||||||||||||||||||||||||||||||||||||
Sequence variations |
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Genome ancestry | Source: PubMed=30894373
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Disease | Neuroblastoma (NCIt: C3270) Neuroblastoma (ORDO: Orphanet_635) | ||||||||||||||||||||||||||||||||||||||||||||||||
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | ||||||||||||||||||||||||||||||||||||||||||||||||
Hierarchy | Parent: CVCL_0531 (SK-N-SH) Children:
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Sex of cell | Female | ||||||||||||||||||||||||||||||||||||||||||||||||
Age at sampling | 4Y | ||||||||||||||||||||||||||||||||||||||||||||||||
Category | Cancer cell line | ||||||||||||||||||||||||||||||||||||||||||||||||
STR profile | Source(s): ATCC; CCRID; CLS; DSMZ; ECACC; Genomics_Center_BCF_Technion; KCLB Markers:
Run an STR similarity search on this cell line | ||||||||||||||||||||||||||||||||||||||||||||||||
Web pages | https://en.wikipedia.org/wiki/SH-SY5Y https://www.atcc.org/en/support/technical-support/faqs/morphology-of-atcc-crl-2266 https://www.proteinatlas.org/learn/cellines http://genome.ucsc.edu/ENCODE/protocols/cell/human/SH-SY5Y_Farnham_protocol.pdf https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/s/cell-lines-detail-101.html https://www.mskcc.org/research-advantage/support/technology/tangible-material/human-neuroblastoma-cell-line-sh-sy5y https://www.phe-culturecollections.org.uk/media/114601/sh-sy5y-cell-line-profile.pdf | ||||||||||||||||||||||||||||||||||||||||||||||||
Publications | PubMed=4748425 PubMed=6137586; DOI=10.1093/jnci/71.4.741 PubMed=3968181; DOI=10.1083/jcb.100.2.588 PubMed=2846152 PubMed=2535691 PubMed=1354203; DOI=10.1002/ijc.2910520116 PubMed=7687927 PubMed=10630978; DOI=10.1667/0033-7587(2000)153[0062:FORIMI]2.0.CO;2 PubMed=11668190; DOI=10.1177/002215540104901105 PubMed=16120276; DOI=10.1016/S1567-7249(01)00007-1 PubMed=14676279; DOI=10.1242/jcs.00834 PubMed=15150091; DOI=10.1158/0008-5472.CAN-03-0809 PubMed=15720811; DOI=10.1593/neo.04310 PubMed=16822308; DOI=10.1186/1471-2407-6-177 PubMed=17506115; DOI=10.1002/nbm.1181 PubMed=18957096; DOI=10.1186/1756-0500-1-95 PubMed=20655465; DOI=10.1016/j.cell.2010.06.004 PubMed=22213050; DOI=10.1002/ijc.27415 PubMed=22460905; DOI=10.1038/nature11003 PubMed=23421552; DOI=10.1089/ast.2012.0822 PubMed=24466371; DOI=10.1593/tlo.13544 PubMed=25182563; DOI=10.1007/s13577-014-0096-9 PubMed=25730103; DOI=10.1021/pr5011718 PubMed=25884760; DOI=10.1371/journal.pcbi.1004130 PubMed=26342562; DOI=10.1016/j.scr.2015.08.008 PubMed=26972028; DOI=10.1016/j.jprot.2016.03.008 PubMed=27141528; DOI=10.1016/j.dib.2016.04.001 PubMed=28118852; DOI=10.1186/s13024-017-0149-0 PubMed=28350380; DOI=10.1038/sdata.2017.33 PubMed=28601559; DOI=10.1016/j.cels.2017.05.009 PubMed=29468137; DOI=10.5501/wjv.v7.i1.10 PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747 PubMed=31068700; DOI=10.1038/s41586-019-1186-3 | ||||||||||||||||||||||||||||||||||||||||||||||||
Cross-references | |||||||||||||||||||||||||||||||||||||||||||||||||
Cell line databases/resources | CLO; CLO_0009015 MCCL; MCC:0000421 CLDB; cl4287 CLDB; cl4288 CLDB; cl4289 CLDB; cl4290 CCRID; 1101HUM-PUMC000026 CCRID; 3101HUMSCSP5014 CCRID; 3101HUMTCHu97 CCRID; 4201PAT-CCTCC00086 CCRID; 4201HUM-CCTCC00210 CCRID; 5301HUM-KCB06107YJ Cell_Model_Passport; SIDM01236 CGH-DB; 63-1 DepMap; ACH-001188 DSMZCellDive; ACC-209 LINCS_LDP; LCL-2000 Lonza; 132 TOKU-E; 3118 | ||||||||||||||||||||||||||||||||||||||||||||||||
Anatomy/cell type resources | BTO; BTO:0000793 | ||||||||||||||||||||||||||||||||||||||||||||||||
Biological sample resources | BioSample; SAMN10987717 ENCODE; ENCBS264AAA | ||||||||||||||||||||||||||||||||||||||||||||||||
Cell line collections (Providers) | Abcam; ab275475 AddexBio; C0005004/63 ATCC; CRL-2266 BCRJ; 0223 CCTCC; GDC0210 CLS; 300154 DSMZ; ACC-209 ECACC; 94030304 ICLC; HTL95013 IZSLER; BS TCL 232 KCB; KCB 2006107YJ KCLB; 22266 Kerafast; ECP004 NCBI_Iran; C611 | ||||||||||||||||||||||||||||||||||||||||||||||||
Chemistry resources | ChEMBL-Cells; CHEMBL3307740 ChEMBL-Targets; CHEMBL614910 PharmacoDB; SHSY5Y_1374_2019 PubChem_Cell_line; CVCL_0019 | ||||||||||||||||||||||||||||||||||||||||||||||||
Encyclopedic resources | Wikidata; Q7390126 | ||||||||||||||||||||||||||||||||||||||||||||||||
Experimental variables resources | EFO; EFO_0002717 |