SH-SY5Y人神经母细胞瘤细胞

价 格:¥1800

期 货:现货(冻存管发2支)

保藏中心:BTCC

资源编号:BTCC-1158

产地:中国

套 餐:

T25/复苏细胞 无血清冻存液 专用完全培养基500ml 南美特级胎牛血清500ml

详细资料说明书下载质检报告(COA)

Cell line nameSH-SY5Y
SynonymsSH-Sy5y; SHSY5Y; SHSY-5Y; SK-SH-SY5Y; SY5Y; SH-SY5Y Parental
AccessionBTCC-1158
Resource Identification InitiativeTo cite this cell line use: SH-SY5Y (BTCC-1158)
CommentsProblematic cell line: Partially contaminated. Some laboratories that are redistributing this cell line are in fact redistributing a contaminated cell line of mouse origin (PubMed=25182563).
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: ENCODE project common cell types; tier 3.
From: Memorial Sloan Kettering Cancer Center; New York; USA.
Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK 810.
Population: Caucasian.
Characteristics: Neuroblastic type (N-type) (PubMed=15720811).
Characteristics: There seem to be differences in the retinoic acid (RA)-induced neuronal phenotype of SH-SY5Y cells from ATCC and ECACC. After 5 days of RA treatment, ECACC cells are slightly larger in size and contains significant amount of neuroblastic (N-type) cells and a small fraction of epithelial (S-type) cells (PubMed=18957096).
Virology: Low susceptibility to infection by Zika virus (ZIKV) (PubMed=29468137).
Doubling time: >55 hours (DSMZ=ACC-209).
Omics: Deep antibody staining analysis.
Omics: Deep exome analysis.
Omics: Deep proteome analysis.
Omics: Deep tyrosine phosphoproteome analysis.
Omics: GPI-anchored proteins analysis by proteomics.
Omics: Mitochondrial proteome analysis by 2D-DE/MS.
Omics: N-glycan profiling.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Misspelling: SH-SY5; Note=Occasionally.
Derived from site: Metastatic; Bone marrow; UBERON=UBERON_0002371.
Sequence variations
  • Mutation; HGNC; 427; ALK; Simple; p.Phe1174Leu (c.3522C>A); ClinVar=VCV000217852; Zygosity=Unspecified (PubMed=28350380).
Genome ancestrySource: PubMed=30894373

Origin% genome


African0
Native American1.17
East Asian, North0.35
East Asian, South0.1
South Asian0.08
European, North69.09
European, South29.2
DiseaseNeuroblastoma (NCIt: C3270)
Neuroblastoma (ORDO: Orphanet_635)
Species of originHomo sapiens (Human) (NCBI Taxonomy: 9606)
HierarchyParent: CVCL_0531 (SK-N-SH)
Children:



CVCL_B0YJ (Abcam SH-SY5Y DLG4 KO)CVCL_B7VN (Abcam SH-SY5Y EFNB2 KO)CVCL_B7VQ (Abcam SH-SY5Y LMOD1 KO)
CVCL_B0YH (Abcam SH-SY5Y PDGFRA KO)CVCL_B0YI (Abcam SH-SY5Y PDGFRB KO)CVCL_B0YK (Abcam SH-SY5Y PINK1 KO)
CVCL_B0N9 (Abcam SH-SY5Y PRKN KO)CVCL_B7VP (Abcam SH-SY5Y SNAP25 KO)CVCL_YJ91 (LINTERNA SH-SY5Y)
CVCL_U924 (SH-SY5Y Rho0 64/5)CVCL_YK67 (SH-SY5Y TagGFP2-SNCA)CVCL_C0U0 (SH-SY5Y-FLAG-NGB)
CVCL_5I52 (SH-SY5Y-HFE)CVCL_5I53 (SH-SY5Y-HFE C282Y)CVCL_5I54 (SH-SY5Y-HFE H63D)
CVCL_C3I6 (SH-SY5Y-parkin clone 1)CVCL_C3I7 (SH-SY5Y-parkin clone 2)CVCL_C3I8 (SH-SY5Y-parkin clone 6)
CVCL_C3I9 (SH-SY5Y-parkin p.A82E)CVCL_C3IA (SH-SY5Y-parkin p.T240R)CVCL_UJ96 (SH-SY5Y-pLNCX2 empty vector)
CVCL_0523 (SH-SY5Y-SPA4CT)CVCL_UJ94 (SH-SY5Y-TrkA, clone P23A)CVCL_UJ95 (SH-SY5Y-TrkB, clone BR6)
CVCL_ZL49 (SH-SY5Y/VCR)CVCL_RR81 (SH-SY5YrCDDP200)CVCL_RS23 (SH-SY5YrCDDP500)
CVCL_RS80 (SH-SY5YrDOX10)CVCL_RS81 (SH-SY5YrDOX20)CVCL_RR53 (SH-SY5YrVCR10)
CVCL_A7QJ (SHSY-5Y Tet-On SNCA)CVCL_VA24 (SHSY5Y-B7H6)CVCL_YJ70 (VAMPIRO SH-SY5Y)
Sex of cellFemale
Age at sampling4Y
CategoryCancer cell line
STR profileSource(s): ATCC; CCRID; CLS; DSMZ; ECACC; Genomics_Center_BCF_Technion; KCLB

Markers:
AmelogeninX
CSF1PO11
D1S165612
D2S44111,11.3
D2S133817,19
D3S135815,16
D5S81812
D6S104312,18
D7S8207,10
D8S117915
D10S124814
D12S39118,22
D13S31711
D16S5398,13
D18S5113,16
D19S43313,14
D21S1131,31.2
D22S104516,17
FGA23.2,24
Penta D10,12
Penta E7,11
TH017,10
TPOX8,11
vWA14,18

Run an STR similarity search on this cell line
Web pageshttps://en.wikipedia.org/wiki/SH-SY5Y
https://www.atcc.org/en/support/technical-support/faqs/morphology-of-atcc-crl-2266
https://www.proteinatlas.org/learn/cellines
http://genome.ucsc.edu/ENCODE/protocols/cell/human/SH-SY5Y_Farnham_protocol.pdf
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/s/cell-lines-detail-101.html
https://www.mskcc.org/research-advantage/support/technology/tangible-material/human-neuroblastoma-cell-line-sh-sy5y
https://www.phe-culturecollections.org.uk/media/114601/sh-sy5y-cell-line-profile.pdf
Publications

PubMed=4748425
Biedler J.L., Helson L., Spengler B.A.
Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture.
Cancer Res. 33:2643-2652(1973)

PubMed=6137586; DOI=10.1093/jnci/71.4.741
Ross R.A., Spengler B.A., Biedler J.L.
Coordinate morphological and biochemical interconversion of human neuroblastoma cells.
J. Natl. Cancer Inst. 71:741-747(1983)

PubMed=3968181; DOI=10.1083/jcb.100.2.588
Meyers M.B., Spengler B.A., Chang T.-D., Melera P.W., Biedler J.L.
Gene amplification-associated cytogenetic aberrations and protein changes in vincristine-resistant Chinese hamster, mouse, and human cells.
J. Cell Biol. 100:588-597(1985)

PubMed=2846152
Preis P.N., Saya H., Nadasdi L., Hochhaus G., Levin V., Sadee W.
Neuronal cell differentiation of human neuroblastoma cells by retinoic acid plus herbimycin A.
Cancer Res. 48:6530-6534(1988)

PubMed=2535691
Ciccarone V., Spengler B.A., Meyers M.B., Biedler J.L., Ross R.A.
Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages.
Cancer Res. 49:219-225(1989)

PubMed=1354203; DOI=10.1002/ijc.2910520116
Gross N., Favre S., Beck D., Meyer M.
Differentiation-related expression of adhesion molecules and receptors on human neuroblastoma tissues, cell lines and variants.
Int. J. Cancer 52:85-91(1992)

PubMed=7687927
Xu Y.-Y., Wands J.R., de la Monte S.M.
Characterization of thread proteins expressed in neuroectodermal tumors.
Cancer Res. 53:3823-3829(1993)

PubMed=10630978; DOI=10.1667/0033-7587(2000)153[0062:FORIMI]2.0.CO;2
Akudugu J.M., Slabbert J.P., Serafin A., Bohm L.
Frequency of radiation-induced micronuclei in neuronal cells does not correlate with clonogenic survival.
Radiat. Res. 153:62-67(2000)

PubMed=11668190; DOI=10.1177/002215540104901105
Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)

PubMed=16120276; DOI=10.1016/S1567-7249(01)00007-1
Scheffler N.K., Miller S.W., Carroll A.K., Anderson C., Davis R.E., Ghosh S.S., Gibson B.W.
Two-dimensional electrophoresis and mass spectrometric identification of mitochondrial proteins from an SH-SY5Y neuroblastoma cell line.
Mitochondrion 1:161-179(2001)

PubMed=14676279; DOI=10.1242/jcs.00834
Goldschneider D., Blanc E., Raguenez G., Barrois M., Legrand A., Le Roux G., Haddada H., Benard J., Douc-Rasy S.
Differential response of p53 target genes to p73 overexpression in SH-SY5Y neuroblastoma cell line.
J. Cell Sci. 117:293-301(2004)

PubMed=15150091; DOI=10.1158/0008-5472.CAN-03-0809
Schaefer K.-L., Brachwitz K., Wai D.H., Braun Y., Diallo R., Korsching E., Eisenacher M., Voss R., van Valen F., Baer C., Selle B., Spahn L., Liao S.-K., Lee K.A.W., Hogendoorn P.C.W., Reifenberger G., Gabbert H.E., Poremba C.
Expression profiling of t(12;22) positive clear cell sarcoma of soft tissue cell lines reveals characteristic up-regulation of potential new marker genes including ERBB3.
Cancer Res. 64:3395-3405(2004)

PubMed=15720811; DOI=10.1593/neo.04310
Walton J.D., Kattan D.R., Thomas S.K., Spengler B.A., Guo H.-F., Biedler J.L., Cheung N.-K.V., Ross R.A.
Characteristics of stem cells from human neuroblastoma cell lines and in tumors.
Neoplasia 6:838-845(2004)

PubMed=16822308; DOI=10.1186/1471-2407-6-177
Dam V., Morgan B.T., Mazanek P., Hogarty M.D.
Mutations in PIK3CA are infrequent in neuroblastoma.
BMC Cancer 6:177.1-177.10(2006)

PubMed=17506115; DOI=10.1002/nbm.1181
Peet A.C., McConville C.M., Wilson M.P., Levine B.A., Reed M., Dyer S.A., Edwards E.C., Strachan M.C., McMullan D.J., Wilkes T.M., Grundy R.G.
1H MRS identifies specific metabolite profiles associated with MYCN-amplified and non-amplified tumour subtypes of neuroblastoma cell lines.
NMR Biomed. 20:692-700(2007)

PubMed=18957096; DOI=10.1186/1756-0500-1-95
Nishida Y., Adati N., Ozawa R., Maeda A., Sakaki Y., Takeda T.
Identification and classification of genes regulated by phosphatidylinositol 3-kinase- and TRKB-mediated signalling pathways during neuronal differentiation in two subtypes of the human neuroblastoma cell line SH-SY5Y.
BMC Res. Notes 1:95.1-95.11(2008)

PubMed=20655465; DOI=10.1016/j.cell.2010.06.004
Holzel M., Huang S.-D., Koster J., Ora I., Lakeman A., Caron H., Nijkamp W., Xie J., Callens T., Asgharzadeh S., Seeger R.C., Messiaen L.M., Versteeg R., Bernards R.
NF1 is a tumor suppressor in neuroblastoma that determines retinoic acid response and disease outcome.
Cell 142:218-229(2010)

PubMed=22213050; DOI=10.1002/ijc.27415
Gawecka J.E., Geerts D., Koster J., Caliva M.J., Sulzmaier F.J., Opoku-Ansah J., Wada R.K., Bachmann A.S., Ramos J.W.
PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma.
Int. J. Cancer 131:1556-1568(2012)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23421552; DOI=10.1089/ast.2012.0822
Zhang Y.-Q., Wang H.-B., Lai C.-J., Wang L., Deng Y.-L.
Comparative proteomic analysis of human SH-SY5Y neuroblastoma cells under simulated microgravity.
Astrobiology 13:143-150(2013)

PubMed=24466371; DOI=10.1593/tlo.13544
Loschmann N., Michaelis M., Rothweiler F., Zehner R., Cinatl J., Voges Y., Sharifi M., Riecken K., Meyer J., von Deimling A., Fichtner I., Ghafourian T., Westermann F., Cinatl J. Jr.
Testing of SNS-032 in a panel of human neuroblastoma cell lines with acquired resistance to a broad range of drugs.
Transl. Oncol. 6:685-696(2013)

PubMed=25182563; DOI=10.1007/s13577-014-0096-9
Jiang T., Wang H.-L.
Identification of cross-contamination in SH-SY5Y cell line.
Hum. Cell 27:176-178(2014)

PubMed=25730103; DOI=10.1021/pr5011718
Hu Y., Mayampurath A., Khan S., Cohen J.K., Mechref Y., Volchenboum S.L.
N-linked glycan profiling in neuroblastoma cell lines.
J. Proteome Res. 14:2074-2081(2015)

PubMed=25884760; DOI=10.1371/journal.pcbi.1004130
Palacios-Moreno J., Foltz L., Guo A.-L., Stokes M.P., Kuehn E.D., George L., Comb M.J., Grimes M.L.
Neuroblastoma tyrosine kinase signaling networks involve FYN and LYN in endosomes and lipid rafts.
PLoS Comput. Biol. 11:e1004130.1-e1004130.33(2015)

PubMed=26342562; DOI=10.1016/j.scr.2015.08.008
Ross R.A., Walton J.D., Han D., Guo H.-F., Cheung N.-K.V.
A distinct gene expression signature characterizes human neuroblastoma cancer stem cells.
Stem Cell Res. 15:419-426(2015)

PubMed=26972028; DOI=10.1016/j.jprot.2016.03.008
Masuishi Y., Kimura Y., Arakawa N., Hirano H.
Identification of glycosylphosphatidylinositol-anchored proteins and omega-sites using TiO2-based affinity purification followed by hydrogen fluoride treatment.
J. Proteomics 139:77-83(2016)

PubMed=27141528; DOI=10.1016/j.dib.2016.04.001
Masuishi Y., Kimura Y., Arakawa N., Hirano H.
Data for identification of GPI-anchored peptides and omega-sites in cancer cell lines.
Data Brief 7:1302-1305(2016)

PubMed=28118852; DOI=10.1186/s13024-017-0149-0
Xicoy H., Wieringa B., Martens G.J.M.
The SH-SY5Y cell line in Parkinson's disease research: a systematic review.
Mol. Neurodegener. 12:10.1-10.11(2017)

PubMed=28350380; DOI=10.1038/sdata.2017.33
Harenza J.L., Diamond M.A., Adams R.N., Song M.M., Davidson H.L., Hart L.S., Dent M.H., Fortina P., Reynolds C.P., Maris J.M.
Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines.
Sci. Data 4:170033-170033(2017)

PubMed=28601559; DOI=10.1016/j.cels.2017.05.009
Bekker-Jensen D.B., Kelstrup C.D., Batth T.S., Larsen S.C., Haldrup C., Bramsen J.B., Sorensen K.D., Hoyer S., Orntoft T.F., Andersen C.L., Nielsen M.L., Olsen J.V.
An optimized shotgun strategy for the rapid generation of comprehensive human proteomes.
Cell Syst. 4:587-599.e4(2017)

PubMed=29468137; DOI=10.5501/wjv.v7.i1.10
Himmelsbach K., Hildt E.
Identification of various cell culture models for the study of Zika virus.
World J. Virol. 7:10-20(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cross-references
Cell line databases/resourcesCLO; CLO_0009015
MCCL; MCC:0000421
CLDB; cl4287
CLDB; cl4288
CLDB; cl4289
CLDB; cl4290
CCRID; 1101HUM-PUMC000026
CCRID; 3101HUMSCSP5014
CCRID; 3101HUMTCHu97
CCRID; 4201PAT-CCTCC00086
CCRID; 4201HUM-CCTCC00210
CCRID; 5301HUM-KCB06107YJ
Cell_Model_Passport; SIDM01236
CGH-DB; 63-1
DepMap; ACH-001188
DSMZCellDive; ACC-209
LINCS_LDP; LCL-2000
Lonza; 132
TOKU-E; 3118
Anatomy/cell type resourcesBTO; BTO:0000793
Biological sample resourcesBioSample; SAMN10987717
ENCODE; ENCBS264AAA
Cell line collections (Providers)Abcam; ab275475
AddexBio; C0005004/63
ATCC; CRL-2266
BCRJ; 0223
CCTCC; GDC0210
CLS; 300154
DSMZ; ACC-209
ECACC; 94030304
ICLC; HTL95013
IZSLER; BS TCL 232
KCB; KCB 2006107YJ
KCLB; 22266
Kerafast; ECP004
NCBI_Iran; C611
Chemistry resourcesChEMBL-Cells; CHEMBL3307740
ChEMBL-Targets; CHEMBL614910
PharmacoDB; SHSY5Y_1374_2019
PubChem_Cell_line; CVCL_0019
Encyclopedic resourcesWikidata; Q7390126
Experimental variables resourcesEFO; EFO_0002717

更多>>相关产品

总部地址:北京市怀柔区渤海镇怀沙路536号

关注我们
在线咨询
在线客服
电话咨询
微信客服
返回顶部