PA-1人卵巢畸胎瘤细胞(STR鉴定正确)

价 格:¥1800

期 货:现货(冻存管发2支)

保藏中心:BTCC

资源编号:BTCC-1336

产地:中国

套 餐:

T25/复苏细胞 无血清冻存液 专用完全培养基500ml 南美特级胎牛血清500ml

详细资料说明书下载质检报告(COA)

Cell line namePA-1
SynonymsPA1; PA I; PAI
AccessionBTCC-1336
Resource Identification InitiativeTo cite this cell line use: PA-1 (BTCC-1336)
CommentsPart of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: KuDOS 95 cell line panel.
Doubling time: 36 hours (PubMed=2653399); ~24 hours (Note=Lot 01212002) (JCRB=JCRB9061); 22.77 hours (GrayJW panel).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Caution: TP53 mutation indicated to be at p.Asn239Asp (c.715A>G) in DOI=10.11418/jtca1981.16.3_173 and PubMed=9359923.
Discontinued: TKG; TKG 0573; true.
Derived from site: Metastatic; Ascites; UBERON=UBERON_0007795.
Sequence variations
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000039648; Zygosity=Heterozygous; Note=Not found in early passages (PubMed=6740333).
  • Mutation; HGNC; 11998; TP53; Simple; p.Pro316Pro (c.948C>T); ClinVar=VCV000750727; Zygosity=Heterozygous (Cosmic-CLP).
HLA typingSource: PubMed=26589293
Class I
HLA-AA*68:02,68:02
HLA-BB*37:04,44:03
HLA-CC*07:01,07:01
Class II
HLA-DQDQB1*04:01,04:01
HLA-DRDRB1*03:02,03:02
Genome ancestrySource: PubMed=30894373

Origin% genome


African21.08
Native American17.69
East Asian, North1.54
East Asian, South0.7
South Asian0.4
European, North26.01
European, South32.58
DiseaseOvarian mixed germ cell tumor (NCIt: C8114)
Mixed germ cell tumor (ORDO: Orphanet_180234)
Species of originHomo sapiens (Human) (NCBI Taxonomy: 9606)
HierarchyChildren:



CVCL_1T15 (222)CVCL_4992 (CH1 [Human ovarian carcinoma])CVCL_3121 (PA-1/6TG-r)
Sex of cellFemale
Age at sampling12Y
CategoryCancer cell line
STR profileSource(s): ATCC; CCRID; CLS; COG; Cosmic-CLP; DepMap; ECACC; JCRB; PubMed=25877200

Markers:
AmelogeninX
CSF1PO9,12
D2S133824
D3S135815
D5S81811
D7S8209
D8S117914,15
D13S3179,10 (ATCC; CCRID; CLS; COG; Cosmic-CLP; DepMap; ECACC; PubMed=25877200)
9,10,13 (JCRB)
D16S5399 (CLS)
9,12 (ATCC; CCRID; COG; Cosmic-CLP; DepMap; ECACC; JCRB; PubMed=25877200)
D18S5115,18
D19S43313
D21S1129,31.2
FGA24
Penta D9,12
Penta E14,20
TH017,9
TPOX11
vWA15,17

Run an STR similarity search on this cell line
Publications

PubMed=6931103; DOI=10.1002/ijc.2910250104
Zeuthen J., Norgaard J.O.R., Avner P., Fellous M., Wartiovaara J., Vaheri A., Rosen A., Giovanella B.C.
Characterization of a human ovarian teratocarcinoma-derived cell line.
Int. J. Cancer 25:19-32(1980)

PubMed=6740333; DOI=10.1126/science.6740333
Tainsky M.A., Cooper C.S., Giovanella B.C., Vande Woude G.F.
An activated rasN gene: detected in late but not early passage human PA1 teratocarcinoma cells.
Science 225:643-645(1984)

PubMed=2653399; DOI=10.1038/bjc.1989.108
Hills C.A., Kelland L.R., Abel G., Siracky J., Wilson A.P., Harrap K.R.
Biological properties of ten human ovarian carcinoma cell lines: calibration in vitro against four platinum complexes.
Br. J. Cancer 59:527-534(1989)

DOI=10.11418/jtca1981.16.3_173
Mihara K., Miyazaki M., Fushimi K., Tsuji T., Inoue Y., Fukaya K.-I., Ohashi R., Namba M.
The p53 gene status and other cellular characteristics of human cell lines maintained in our laboratory.
Tissue Cult. Res. Commun. 16:173-178(1997)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::aid-mc5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9359923; DOI=10.18926/AMO/30789
Mihara K., Miyazaki M., Kondo T., Fushimi K., Tsuji T., Inoue Y., Fukaya K.-I., Ishioka C., Namba M.
Yeast functional assay of the p53 gene status in human cell lines maintained in our laboratory.
Acta Med. Okayama 51:261-265(1997)

PubMed=9698466; DOI=10.1006/gyno.1998.5039
Maxwell G.L., Risinger J.I., Tong B., Shaw H., Barrett J.C., Berchuck A., Futreal P.A.
Mutation of the PTEN tumor suppressor gene is not a feature of ovarian cancers.
Gynecol. Oncol. 70:13-16(1998)

PubMed=10567668; DOI=10.3892/ijmm.4.6.597
Gao C., Miyazaki M., Li J.W., Tsuji T., Inoue Y., Namba M.
Cytogenetic characteristics and p53 gene status of human teratocarcinoma PA-1 cells in 407-445 passages.
Int. J. Mol. Med. 4:597-600(1999)

PubMed=11314036; DOI=10.1038/sj.onc.1204211
Forgacs E., Wren J.D., Kamibayashi C., Kondo M., Xu X.L., Markowitz S.D., Tomlinson G.E., Muller C.Y., Gazdar A.F., Garner H.R., Minna J.D.
Searching for microsatellite mutations in coding regions in lung, breast, ovarian and colorectal cancers.
Oncogene 20:1005-1009(2001)

PubMed=11330945; DOI=10.1006/gyno.2001.6132
Watanabe T., Imoto I., Kosugi Y., Ishiwata I., Inoue S., Takayama M., Sato A., Inazawa J.
A novel amplification at 17q21-23 in ovarian cancer cell lines detected by comparative genomic hybridization.
Gynecol. Oncol. 81:172-177(2001)

PubMed=15677628; DOI=10.1093/carcin/bgi032
Gorringe K.L., Chin S.-F., Pharoah P.D.P., Staines J.M., Oliveira C., Edwards P.A.W., Caldas C.
Evidence that both genetic instability and selection contribute to the accumulation of chromosome alterations in cancer.
Carcinogenesis 26:923-930(2005)

PubMed=16080959; DOI=10.1016/j.cancergencyto.2005.01.003
Sarraf S., Tejada R., Abawi M., Oberst M., Dennis T., Simon K.C., Blancato J.
The human ovarian teratocarcinoma cell line PA-1 demonstrates a single translocation: analysis with fluorescence in situ hybridization, spectral karyotyping, and bacterial artificial chromosome microarray.
Cancer Genet. Cytogenet. 161:63-69(2005)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=22710073; DOI=10.1016/j.ygyno.2012.06.017
Korch C.T., Spillman M.A., Jackson T.A., Jacobsen B.M., Murphy S.K., Lessey B.A., Jordan V.C., Bradford A.P.
DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.
Gynecol. Oncol. 127:241-248(2012)

PubMed=23415752; DOI=10.1016/j.molonc.2012.12.007
Stordal B., Timms K., Farrelly A., Gallagher D., Busschots S., Renaud M., Thery J., Williams D., Potter J., Tran T., Korpanty G., Cremona M., Carey M.S., Li J., Li Y., Aslan O., O'Leary J.J., Mills G.B., Hennessy B.T.
BRCA1/2 mutation analysis in 41 ovarian cell lines reveals only one functionally deleterious BRCA1 mutation.
Mol. Oncol. 7:567-579(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line databases/resourcesCLO; CLO_0008350
MCCL; MCC:0000377
CLDB; cl3856
CLDB; cl3857
CLDB; cl3858
CLDB; cl3859
CCRID; 1101HUM-PUMC000021
Cell_Model_Passport; SIDM01140
CGH-DB; 9358-4
Cosmic-CLP; 909255
DepMap; ACH-001374
LINCS_LDP; LCL-1416
Anatomy/cell type resourcesBTO; BTO:0001591
Biological sample resourcesBioSample; SAMN01821586
BioSample; SAMN03473209
BioSample; SAMN03472415
Cell line collections (Providers)ATCC; CRL-1572
BCRC; 60063
CLS; 300402
ECACC; 90013101
ICLC; HTL97002
IZSLER; BS TCL 179
JCRB; JCRB9061
RCB; RCB1946 - Discontinued
TKG; TKG 0573
Chemistry resourcesChEMBL-Cells; CHEMBL3308812
ChEMBL-Targets; CHEMBL614949
GDSC; 909255
PharmacoDB; PA1_1235_2019
PubChem_Cell_line; CVCL_0479
Encyclopedic resourcesWikidata; Q54937331
Experimental variables resourcesEFO; EFO_0006465

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