C2C12小鼠成肌细胞

价 格:¥1800

期 货:现货(冻存管发2支)

保藏中心:BTCC

资源编号:BTCC-2006

产地:中国

套 餐:

T25/复苏细胞 无血清冻存液 专用完全培养基500ml 南美特级胎牛血清500ml

详细资料说明书下载质检报告(COA)

Cell line nameC2C12
SynonymsC2c12; C2-C12; C12
AccessionBTCC-2006
Resource Identification InitiativeTo cite this cell line use: C2C12 (BTCC-2006)
CommentsPart of: ENCODE project mouse cell lines.
Characteristics: Capable, upon starvation, of differentiating into contractile myotubes that express muscle-specific proteins.
Doubling time: 19.6 +- 0.4 hours (Note=At 22th passage), 18.4 +- 0.2 (Note=At 38th passage) (PubMed=22020321); ~20 hours (DSMZ=ACC-565).
Omics: Cell surface proteome.
Omics: Deep proteome analysis.
Omics: H3K27me3 ChIP-seq epigenome analysis.
Omics: H3K36me3 ChIP-seq epigenome analysis.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: H3K79me2 ChIP-seq epigenome analysis.
Omics: H3K79me3 ChIP-seq epigenome analysis.
Omics: Metabolome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Misspelling: C1C12; Note=Occasionally.
Derived from site: In situ; Skeletal muscle; UBERON=UBERON_0001134.
Cell type: Myoblast; CL=CL_0000056.
Species of originMus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C3H.
HierarchyParent: CVCL_6812 (C2 [Mouse myoblast])
Children:



CVCL_AS32 (C2C12 Clone 5 DYSF shRNA)CVCL_UR38 (C2C12 Scrambled)CVCL_RQ02 (C2C12-Bmpr1b)
CVCL_B0JA (C2C12-BRE)CVCL_A0NQ (C2C12-hITGA11)CVCL_RQ03 (C2C12-R486Q-Bmpr1b)
CVCL_RQ04 (C2C12-R486W-Bmpr1b)CVCL_KS37 (C2C12/NFkB-luc)CVCL_VR09 (C2C12i)
CVCL_0189 (C2C12N)CVCL_A6JR (ChR2-C2C12)CVCL_3900 (COM-3)
CVCL_3902 (INC-2)CVCL_KW18 (PathHunter C2C12 CXCR4 beta-arrestin)CVCL_KW19 (PathHunter C2C12 CXCR5 beta-arrestin)
CVCL_KW20 (PathHunter C2C12 KISS1R beta-arrestin)CVCL_KW21 (PathHunter C2C12 TACR1 beta-arrestin)
Sex of cellFemale
Age at sampling2M
CategorySpontaneously immortalized cell line
Web pageshttps://en.wikipedia.org/wiki/C2C12
https://www.phe-culturecollections.org.uk/media/157858/c2c12-cell-line-profile.pdf
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/c/cell-lines-detail-123.html
https://web.archive.org/web/20130502024425/http://www.stanford.edu/group/blau/protocols/c2lineprotocol.html
Publications

PubMed=563524; DOI=10.1038/270725a0
Yaffe D., Saxel O.
Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle.
Nature 270:725-727(1977)

PubMed=8023908; DOI=10.1152/ajpcell.1994.266.6.C1795
McMahon D.K., Anderson P.A.W., Nassar R., Bunting J.B., Saba Z., Oakeley A.E., Malouf N.N.
C2C12 cells: biophysical, biochemical, and immunocytochemical properties.
Am. J. Physiol. 266:C1795-C1802(1994)

PubMed=15596414
Burattini S., Ferri P., Battistelli M., Curci R., Luchetti F., Falcieri E.
C2C12 murine myoblasts as a model of skeletal muscle development: morpho-functional characterization.
Eur. J. Histochem. 48:223-233(2004)

PubMed=19298647; DOI=10.1186/1471-2121-10-20
Herrera B., Inman G.J.
A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
BMC Cell Biol. 10:20.1-20.11(2009)

PubMed=19350625; DOI=10.1002/bit.22318
Fujita H., Shimizu K., Nagamori E.
Novel method for fabrication of skeletal muscle construct from the C2C12 myoblast cell line using serum-free medium AIM-V.
Biotechnol. Bioeng. 103:1034-1041(2009)

PubMed=20635352; DOI=10.1002/bit.22865
Fujita H., Endo A., Shimizu K., Nagamori E.
Evaluation of serum-free differentiation conditions for C2C12 myoblast cells assessed as to active tension generation capability.
Biotechnol. Bioeng. 107:894-901(2010)

PubMed=22020321; DOI=10.1016/j.yexcr.2011.10.004
Humphrey G.W., Mekhedov E., Blank P.S., de Morree A., Pekkurnaz G., Nagaraju K., Zimmerberg J.
GREG cells, a dysferlin-deficient myogenic mouse cell line.
Exp. Cell Res. 318:127-135(2012)

PubMed=24735950; DOI=10.1016/j.scr.2014.03.004
Willkomm L., Schubert S., Jung R., Elsen M., Borde J., Gehlert S., Suhr F., Bloch W.
Lactate regulates myogenesis in C2C12 myoblasts in vitro.
Stem Cell Res. 12:742-753(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=25894527; DOI=10.1371/journal.pone.0121314
Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A.S., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P.W., Wollscheid B.
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:E0121314-E0121314(2015)

Cross-references
Cell line databases/resourcesCLO; CLO_0002071
CLO; CLO_0050871
MCCL; MCC:0000079
CLDB; cl563
CLDB; cl564
CLDB; cl5172
CCRID; 1101MOU-PUMC000099
CCRID; 3101MOUGNM26
CCRID; 3101MOUSCSP505
CCRID; 4201MOU-CCTCC00175
DSMZCellDive; ACC-565
FCS-free; 221-5-486-3-3
FCS-free; 221-5-527-1-3-3
FCS-free; 221-17-457-3-4-3
FCS-free; 221-17-505-3-4-12
FCS-free; 221-21-488-3-4-12
Lonza; 692
TOKU-E; 728
Anatomy/cell type resourcesBTO; BTO:0000165
Biological sample resourcesENCODE; ENCBS124ENC
ENCODE; ENCBS299ZOZ
ENCODE; ENCBS981MXJ
Cell line collections (Providers)Abcam; ab279976
AddexBio; P0028001/4665
ATCC; CRL-1772
BCRC; 60083
BCRJ; 0058
CCTCC; GDC0175
CLS; 400476
DSMZ; ACC-565
ECACC; 91031101
IZSLER; BS CL 148
KCB; KCB 2012115YJ
NCBI_Iran; C521
RCB; RCB0987
Chemistry resourcesChEMBL-Cells; CHEMBL3308003
ChEMBL-Targets; CHEMBL612655
PubChem_Cell_line; CVCL_0188
Encyclopedic resourcesWikidata; Q5008075
Experimental variables resourcesEFO; EFO_0001098

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