DH82狗肾恶性组织细胞增生症细胞

价 格:¥1800

期 货:现货(冻存管发2支)

保藏中心:BTCC

资源编号:BTCC-4022

产地:中国

套 餐:

T25/复苏细胞 无血清冻存液 专用完全培养基500ml 南美特级胎牛血清500ml

详细资料说明书下载质检报告(COA)

Cell line nameDH82
SynonymsDH-82; DH 82
AccessionBTCC-4022
Resource Identification InitiativeTo cite this cell line use: DH82 (BTCC-4022)
CommentsPart of: FACC canine tumor cell line panel.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10389.
Characteristics: Can be infected by Trypanosoma cruzi which can then replicate and differentiate inside the cell.
Characteristics: Susceptible to infection by Ehrlichia ruminantium (PubMed=11585095; PubMed=12234001).
Virology: Supports the replication of equine infectious anemia virus (EIAV) (PubMed=11377712).
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Doubling time: 26 hours (PubMed=27257868).
Omics: Deep exome analysis.
Omics: Transcriptome analysis by microarray.
Sequence variations
  • Mutation; VGNC; 45168; PTPN11; Simple; p.Gly503Val (c.1508G>T); Zygosity=Homozygous (PubMed=31175136).
DiseaseCanine histiocytic sarcoma (NCIt: C128125)
Species of originCanis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Golden Retriever.
HierarchyChildren:

CVCL_3461 (DH82ECOK)
Sex of cellMale
Age at sampling10Y
CategoryCancer cell line
STR profileSource(s): PubMed=27197945; PubMed=31175136

Markers:
Dog FHC2010231
Dog FHC2054156,168
Dog FHC2079275
Dog PEZ1119,123
Dog PEZ12270,285
Dog PEZ20176
Dog PEZ3121
Dog PEZ5107
Dog PEZ6179
Dog PEZ8232,240

Run an STR similarity search on this cell line
Web pageshttps://web.archive.org/web/20201109215324/www.infarktforschung.de/macrophages_cell_lines.html
Publications

PubMed=3350786; DOI=10.1007/BF02623551
Wellman M.L., Krakowka S., Jacobs R.M., Kociba G.J.
A macrophage-monocyte cell line from a dog with malignant histiocytosis.
In Vitro Cell. Dev. Biol. 24:223-229(1988)

Patent=US5192679
Dawson J.E., Rikihisa Y.
Growing Ehrlichia species in a continuous cell line.
Patent number US5192679, 09-Mar-1993

PubMed=11377712; DOI=10.1016/s0166-0934(01)00288-9
Hines R., Maury W.
DH82 cells: a macrophage cell line for the replication and study of equine infectious anemia virus.
J. Virol. Methods 95:47-56(2001)

PubMed=11585095
Zweygarth E., Josemans A.I.
Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line.
Onderstepoort J. Vet. Res. 68:155-157(2001)

PubMed=12234001
Zweygarth E., Josemans A.I., Van Strijp M.F., Van Heerden H., Allsopp M.T.E.P., Allsopp B.A.
The Kumm isolate of Ehrlichia ruminantium: in vitro isolation, propagation and characterization.
Onderstepoort J. Vet. Res. 69:147-153(2002)

PubMed=21908323; DOI=10.1177/1040638711408064
O'Donoghue L.E., Rivest J.P., Duval D.L.
Polymerase chain reaction-based species verification and microsatellite analysis for canine cell line validation.
J. Vet. Diagn. Invest. 23:780-785(2011)

PubMed=25534080; DOI=10.1016/j.vetimm.2014.11.006
Heinrich F., Contioso V.B., Stein V.M., Carlson R., Tipold A., Ulrich R., Puff C., Baumgartner W., Spitzbarth I.
Passage-dependent morphological and phenotypical changes of a canine histiocytic sarcoma cell line (DH82 cells).
Vet. Immunol. Immunopathol. 163:86-92(2015)

PubMed=27257868; DOI=10.1371/journal.pone.0156689
Maeda J., Froning C.E., Brents C.A., Rose B.J., Thamm D.H., Kato T.A.
Intrinsic radiosensitivity and cellular characterization of 27 canine cancer cell lines.
PLoS ONE 11:E0156689-E0156689(2016)

PubMed=26842912; DOI=10.1111/vco.12197
Hartley G., Faulhaber E., Caldwell A., Coy J., Kurihara J., Guth A., Regan D., Dow S.
Immune regulation of canine tumour and macrophage PD-L1 expression.
Vet. Comp. Oncol. 15:534-549(2017)

PubMed=27197945; DOI=10.1111/vco.12192
Fowles J.S., Dailey D.D., Gustafson D.L., Thamm D.H., Duval D.L.
The Flint Animal Cancer Center (FACC) canine tumour cell line panel: a resource for veterinary drug discovery, comparative oncology and translational medicine.
Vet. Comp. Oncol. 15:481-492(2017)

PubMed=28620374; DOI=10.3389/fimmu.2017.00604
Mendonca P.H.B., da Rocha R.F.D.B., de Braz Moraes J.B., LaRocque-de-Freitas I.F., Logullo J., Morrot A., Nunes M.P., Freire-de-Lima C.G., Decote-Ricardo D.
Canine macrophage DH82 cell line as a model to study susceptibility to Trypanosoma cruzi infection.
Front. Immunol. 8:604.1-604.11(2017)

PubMed=31175136; DOI=10.1158/1535-7163.MCT-18-1346
Das S., Idate R., Cronise K.E., Gustafson D.L., Duval D.L.
Identifying candidate druggable targets in canine cancer cell lines using whole exome sequencing.
Mol. Cancer Ther. 18:1460-1471(2019)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cross-references
Cell line databases/resourcesCLO; CLO_0002775
CLDB; cl1058
CLDB; cl1059
CCRID; 1101CAN-PUMC000818
CCRID; 3101CANTCO3
Cell line collections (Providers)ATCC; CRL-10389
BCRC; 60110
BCRJ; 0077
CLS; 305003
ECACC; 94062922
ICLC; ATL96008
KCB; KCB 2014070YJ
Encyclopedic resourcesWikidata; Q54831065
Gene expression databasesGEO; GSM1974787

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