IPEC-J2猪小肠上皮细胞

价 格:¥1800

期 货:现货(冻存管发2支)

保藏中心:BTCC

资源编号:BTCC-4025

产地:中国

套 餐:

T25/复苏细胞 无血清冻存液 专用完全培养基500ml 南美特级胎牛血清500ml

详细资料说明书下载质检报告(COA)

Cell line nameIPEC-J2
SynonymsIPECJ2; Intestinal Porcine Epithelial Cell line-J2
AccessionBTCC-4025
Resource Identification InitiativeTo cite this cell line use: IPEC-J2 (BTCC-4025)
CommentsCharacteristics: Undergoes a process of spontaneous differentiation that leads to the formation of a polarized monolayer with low or high transepithelial electrical resistance (TEER), depending on the type of serum added to the culture medium, within 1-2 weeks (PubMed=29787056).
Virology: Susceptible to infection by porcine circovirus type 2 (PCV2) (PubMed=25407967).
Doubling time: ~40-60 hours (DSMZ=ACC-701).
Omics: Deep proteome analysis.
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Small intestine, jejunum; UBERON=UBERON_0002115.
Cell type: Enterocyte; CL=CL_0000584.
Species of originSus scrofa (Pig) (NCBI Taxonomy: 9823)
HierarchyChildren:



CVCL_6C41 (IPEC-J2 MDR1)CVCL_A9M7 (IPEC-J2 rMDR1a)CVCL_6C22 (IPEC-J2-3)
CVCL_6C23 (IPEC-J2-9)CVCL_ZZ35 (SD-PJEC)
Sex of cellSex unspecified
Age at sampling<1D
CategorySpontaneously immortalized cell line
Publications

DOI=10.1016/S0016-5085(89)80002-2
Berschneider H.M.
Development of normal cultured small intestinal epithelial cell lines which transport Na and Cl.
Gastroenterology 96:A41-A41(1989)

PubMed=16215741; DOI=10.1007/s00418-005-0067-z
Schierack P., Nordhoff M., Pollmann M., Weyrauch K.D., Amasheh S., Lodemann U., Jores J., Tachu B., Kleta S., Blikslager A., Tedin K., Wieler L.H.
Characterization of a porcine intestinal epithelial cell line for in vitro studies of microbial pathogenesis in swine.
Histochem. Cell Biol. 125:293-305(2006)

PubMed=21626283; DOI=10.1007/s10616-011-9362-9
Geens M.M., Niewold T.A.
Optimizing culture conditions of a porcine epithelial cell line IPEC-J2 through a histological and physiological characterization.
Cytotechnology 63:415-423(2011)

PubMed=22074860; DOI=10.1016/j.vetmic.2011.10.017
Brosnahan A.J., Brown D.R.
Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations.
Vet. Microbiol. 156:229-237(2012)

PubMed=22193509; DOI=10.1007/s10616-011-9420-3
Steube K.G., Koelz A.-L., Uphoff C.C., Drexler H.G., Kluess J., Steinberg P.
The necessity of identity assessment of animal intestinal cell lines: a case report.
Cytotechnology 64:373-378(2012)

DOI=10.1155/2013/980651
Stoy A.C.F., Heegaard P.M.H., Sangild P.T., Ostergaard M.V., Skovgaard K.
Gene expression analysis of the IPEC-J2 cell line: a simple model for the inflammation-sensitive preterm intestine.
ISRN Genomics 2013:980651.1-980651.7(2013)

PubMed=24260272; DOI=10.1371/journal.pone.0079643
Zakrzewski S.S., Richter J.F., Krug S.M., Jebautzke B., Lee I.-F.M., Rieger J., Sachtleben M., Bondzio A., Schulzke J.-D., Fromm M., Gunzel D.
Improved cell line IPEC-J2, characterized as a model for porcine jejunal epithelium.
PLoS ONE 8:E79643-E79643(2013)

PubMed=25407967; DOI=10.1186/s12985-014-0193-0
Yan M.-F., Zhu L.-Q., Yang Q.
Infection of porcine circovirus 2 (PCV2) in intestinal porcine epithelial cell line (IPEC-J2) and interaction between PCV2 and IPEC-J2 microfilaments.
Virol. J. 11:193.1-193.9(2014)

PubMed=26147118; DOI=10.1371/journal.pone.0132323
Nossol C., Barta-Boszormenyi A., Kahlert S., Zuschratter W., Faber-Zuschratter H., Reinhardt N., Ponsuksili S., Wimmers K., Diesing A.-K., Rothkotter H.-J.
Comparing two intestinal porcine epithelial cell lines (IPECs): morphological differentiation, function and metabolism.
PLoS ONE 10:E0132323-E0132323(2015)

PubMed=29787056; DOI=10.1007/978-3-319-16104-4_12
Vergauwen H.
The IPEC-J2 cell line.
(In) The impact of food bioactives on health. In vitro and ex vivo models; Verhoeckx K., Cotter P., Lopez-Exposito I., Kleiveland C., Lea T., Mackie A., Requena T., Swiatecka D., Wichers H. (eds.); pp.125-134; Springer; Cham (2015)

PubMed=26651362; DOI=10.1021/acs.molpharmaceut.5b00874
Saaby L., Helms H.C., Brodin B.
IPEC-J2 MDR1, a novel high-resistance cell line with functional expression of human P-glycoprotein (ABCB1) for drug screening studies.
Mol. Pharm. 13:640-652(2016)

PubMed=26975772; DOI=10.4014/jmb.1512.12075
Kim S.H., Pajarillo E.A.B., Balolong M.P., Lee J.Y., Kang D.-K.
Constructing proteome reference map of the porcine jejunal cell line (IPEC-J2) by label-free mass spectrometry.
J. Microbiol. Biotechnol. 26:1124-1131(2016)

PubMed=32708885; DOI=10.3390/pharmaceutics12070673
Saaby L., Trasborg J., Rasmussen M.A., Holst B., Brodin B.
IPEC-J2 rMdr1a, a new cell line with functional expression of rat P-glycoprotein encoded by rat Mdr1a for drug screening purposes.
Pharmaceutics 12:673.1-673.17(2020)

Cross-references
Cell line databases/resourcesDSMZCellDive; ACC-701
Anatomy/cell type resourcesBTO; BTO:0006402
Cell line collections (Providers)DSMZ; ACC-701
IZSLER; BS CL 205
Encyclopedic resourcesWikidata; Q54897969
Gene expression databasesGEO; GSM1646398
GEO; GSM1646399
GEO; GSM1646400

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