BEAS-2B人支气管上皮细胞

价 格:¥2400

期 货:现货(冻存管发2支)

保藏中心:BTCC

资源编号:BTCC-1021

产地:中国

套 餐:

T25/复苏细胞 无血清冻存液 专用完全培养基500ml 南美特级胎牛血清500ml

详细资料说明书下载质检报告(COA)

Cell line nameBEAS-2B
SynonymsBeas-2B; BEAS 2B; BEAS2B; Beas2B; Bronchial Epithelium transformed with Ad12-SV40 2B
AccessionBTCC-1021
Resource Identification InitiativeTo cite this cell line use: BEAS-2B (BTCC-1021)
CommentsGroup: Patented cell line.
Part of: Small cell lung cancer p53 hotspot mutation cell panel (ATCC TCP-2040).
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-9609.
Transformant: Ad12-SV40 hybrid virus.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Bronchus, epithelium; UBERON=UBERON_0002031.
Species of originHomo sapiens (Human) (NCBI Taxonomy: 9606)
HierarchyChildren:



CVCL_4400 (BBM)CVCL_C2UN (BEAS-2B IRF1 KO)CVCL_LJ64 (BEAS-2B/c-myc)
CVCL_LJ65 (BEAS-2B/c-raf-1)CVCL_LJ66 (BEAS-2B/c-raf-1/c-myc)CVCL_WZ50 (BEAS-2B/CYP1A2)
CVCL_WZ48 (BEAS-2B/CYP2A13)CVCL_WZ49 (BEAS-2B/CYP2A6)CVCL_LJ61 (BEAS-2B/EJ-ras)
CVCL_LJ62 (BEAS-2B/N-ras)CVCL_UR57 (BEAS-2B/R1)CVCL_LJ63 (BEAS-2B/S6)
CVCL_LJ68 (BERB)CVCL_LJ71 (Bp53MT143)CVCL_LJ70 (Bp53WT)
CVCL_LJ60 (BVK-Tll)CVCL_3658 (BZR)
Originate from same individualCVCL_0171 ! BET-1A
Sex of cellMale
CategoryTransformed cell line
STR profileSource(s): ATCC; CCRID; ECACC; PubMed=25877200

Markers:
AmelogeninX,Y
CSF1PO9,12
D2S133822,23
D3S135815,17
D5S81812,13
D7S82010,13
D8S117913,15
D13S31713
D16S53911,12 (PubMed=25877200)
12 (ATCC; CCRID; ECACC)
D18S5118,19
D19S43313.2 (PubMed=25877200)
13.2,15.2 (CCRID)
D21S1128,30
FGA20,24
Penta D2.2,13
Penta E5,8
TH017,9.3
TPOX6,11
vWA17,18

Run an STR similarity search on this cell line
Web pageshttps://www.atcc.org/en/support/technical-support/faqs/crl-9609-growth-and-morphology
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/b/cell-lines-detail-39.html
Publications

PubMed=2450641
Reddel R.R., Ke Y., Gerwin B.I., McMenamin M.G., Lechner J.F., Su R.T., Brash D.E., Park J.-B., Rhim J.S., Harris C.C.
Transformation of human bronchial epithelial cells by infection with SV40 or adenovirus-12 SV40 hybrid virus, or transfection via strontium phosphate coprecipitation with a plasmid containing SV40 early region genes.
Cancer Res. 48:1904-1909(1988)

PubMed=2846394; DOI=10.1111/j.1432-0436.1988.tb00592.x
Ke Y., Reddel R.R., Gerwin B.I., Miyashita M., McMenamin M.G., Lechner J.F., Harris C.C.
Human bronchial epithelial cells with integrated SV40 virus T antigen genes retain the ability to undergo squamous differentiation.
Differentiation 38:60-66(1988)

Patent=US4885238
Reddel R.R., Yang K., Rhim J.S., Brash D.E., Su R.T., Lechner J.F., Gerwin B.I., Harris C.C., Amstad P.
Immortalized human bronchial epithelial mesothelial cell lines.
Patent number US4885238, 05-Dec-1989

PubMed=2539488; DOI=10.1093/jnci/81.8.587
Bonfil R.D., Reddel R.R., Ura H., Reich R., Fridman R., Harris C.C., Klein-Szanto A.J.P.
Invasive and metastatic potential of a v-Ha-ras-transformed human bronchial epithelial cell line.
J. Natl. Cancer Inst. 81:587-594(1989)

DOI=10.1007/978-1-4612-0411-4_25
Lehman T.A., Harris C.C.
Neoplastic transformation and suppression of transformation of human bronchial epithelial cells in vitro.
(In) Neoplastic transformation in human cell system. Mechanism of carcinogenesis; Rhim J.S., Dritschilo A. (eds.); pp.251-258; Humana Press; New York (1990)

PubMed=8504475; DOI=10.1093/carcin/14.5.833
Lehman T.A., Modali R., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C.
p53 mutations in human immortalized epithelial cell lines.
Carcinogenesis 14:833-839(1993)

PubMed=7972006; DOI=10.1073/pnas.91.23.11045
Okamoto A., Demetrick D.J., Spillare E.A., Hagiwara K., Hussain S.P., Bennett W.P., Forrester K., Gerwin B.I., Serrano M., Beach D.H., Harris C.C.
Mutations and altered expression of p16INK4 in human cancer.
Proc. Natl. Acad. Sci. U.S.A. 91:11045-11049(1994)

Patent=US5506131
Harris C.C., Gelboin H.V., Gonzalez F.J., Mace K.C., Pfeifer A.M.A.
Immortalized human cell lines containing exogenous cytochrome P450 genes.
Patent number US5506131, 09-Apr-1996

PubMed=12894236; DOI=10.1038/sj.onc.1206728
Ma Y., Koza-Taylor P.H., DiMattia D.A., Hames L., Fu H.-N., Dragnev K.H., Turi T., Beebe J.S., Freemantle S.J., Dmitrovsky E.
Microarray analysis uncovers retinoid targets in human bronchial epithelial cells.
Oncogene 22:4924-4932(2003)

PubMed=15463957; DOI=10.1016/j.jcf.2004.05.040
Gruenert D.C., Willems M., Cassiman J.-J., Frizzell R.A.
Established cell lines used in cystic fibrosis research.
J. Cyst. Fibros. 3:191-196(2004)

PubMed=17331233; DOI=10.1186/1471-2164-8-62
Nymark P., Lindholm P.M., Korpela M.V., Lahti L., Ruosaari S., Kaski S., Hollmen J., Anttila S., Kinnula V.L., Knuutila S.
Gene expression profiles in asbestos-exposed epithelial and mesothelial lung cell lines.
BMC Genomics 8:62.1-62.14(2007)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22521957; DOI=10.1016/j.taap.2012.04.003
Stueckle T.A., Lu Y., Davis M.E., Wang L., Jiang B.-H., Holaskova I., Schafer R., Barnett J.B., Rojanasakul Y.
Chronic occupational exposure to arsenic induces carcinogenic gene signaling networks and neoplastic transformation in human lung epithelial cells.
Toxicol. Appl. Pharmacol. 261:204-216(2012)

PubMed=25524072; DOI=10.1002/jat.3094
Zhao F., Klimecki W.T.
Culture conditions profoundly impact phenotype in BEAS-2B, a human pulmonary epithelial model.
J. Appl. Toxicol. 35:945-951(2015)

PubMed=31156620; DOI=10.3389/fimmu.2019.01019
Panda D., Gjinaj E., Bachu M., Squire E., Novatt H., Ozato K., Rabin R.L.
IRF1 maintains optimal constitutive expression of antiviral genes and regulates the early antiviral response.
Front. Immunol. 10:1019.1-1019.16(2019)

PubMed=31900469; DOI=10.1371/journal.pone.0227174
Han X.-Y., Na T., Wu T.-T., Yuan B.-Z.
Human lung epithelial BEAS-2B cells exhibit characteristics of mesenchymal stem cells.
PLoS ONE 15:E0227174-E0227174(2020)

Cross-references
Cell line databases/resourcesCLO; CLO_0001925
MCCL; MCC:0000060
CCRID; 3101HUMSCSP5067
CCRID; 5301HUM-KCB09022YJ
Lonza; 343
Anatomy/cell type resourcesBTO; BTO:0002923
Biological sample resourcesBioSample; SAMN03471073
BioSample; SAMN03472862
Cell line collections (Providers)AddexBio; T0007001/26
ATCC; CRL-9609
BCRJ; 0395
CCTCC; GDC0139
CLS; 300311
ECACC; 95102433
KCB; KCB 200922YJ
Kerafast; ENH021-FP
NCBI_Iran; C561
Chemistry resourcesChEMBL-Cells; CHEMBL4295386
ChEMBL-Targets; CHEMBL4296403
PubChem_Cell_line; CVCL_0168
Encyclopedic resourcesWikidata; Q54795940
Experimental variables resourcesEFO; EFO_0001089
Gene expression databasesGEO; GSE33520
GEO; GSM139654
GEO; GSM139655
GEO; GSM139656
GEO; GSM139657
GEO; GSM139659
GEO; GSM139660
GEO; GSM139661
GEO; GSM139663
GEO; GSM139664
GEO; GSM139666
GEO; GSM253389
GEO; GSM827258
GEO; GSM3138797
GEO; GSM3138798
GEO; GSM3138799
GEO; GSM3138800
GEO; GSM3138801
GEO; GSM3138802
GEO; GSM3138803
GEO; GSM3138804
GEO; GSM3138805
Polymorphism and mutation databasesIARC_TP53; 23556
Progenetix; CVCL_0168
Proteomic databasesPRIDE; PXD003503
Entry history
Entry creation04-Apr-2012
Last entry update29-Jun-2023
Version number33

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