Caco-2人结直肠腺癌细胞

价 格:¥2400

期 货:现货(冻存管发2支)

保藏中心:BTCC

资源编号:BTCC-1033

产地:中国

套 餐:

T25/复苏细胞 无血清冻存液 专用完全培养基500ml 南美特级胎牛血清500ml

详细资料说明书下载质检报告(COA)

Cell line nameCaco-2
SynonymsCaCo-2; CACO-2; Caco 2; CACO 2; CACO2; CaCo2; CaCO2; Caco2; Caco-2/ATCC; Caco-II
AccessionBTCC-1033
Resource Identification InitiativeTo cite this cell line use: Caco-2 (BTCC-1033)
CommentsPart of: AstraZeneca Colorectal cell line (AZCL) panel.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: ENCODE project common cell types; tier 3.
Part of: MD Anderson Cell Lines Project.
Population: Caucasian.
Virology: Highly susceptible to infection by SARS coronavirus (SARS-CoV) (PubMed=15316659).
Virology: Susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257; PubMed=34339474).
Doubling time: 51.41 hours (PubMed=25944804); 3-4 days (ATCC=HTB-37); ~60-70 hours (CLS=300137); ~80 hours (DSMZ=ACC-169); ~32 hours (PBCF).
Microsatellite instability: Stable (MSS) (PubMed=24042735; PubMed=24755471; PubMed=25926053; PubMed=28683746).
Omics: CTCF ChIP-seq epigenome analysis.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: H3K27me3 ChIP-seq epigenome analysis.
Omics: H3K36me3 ChIP-seq epigenome analysis.
Omics: Deep antibody staining analysis.
Omics: Deep exome analysis.
Omics: Deep phosphoproteome analysis.
Omics: Deep proteome analysis.
Omics: miRNA expression profiling.
Omics: N-glycan profiling.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Omics: Transcriptome analysis by serial analysis of gene expression (SAGE).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; 583; APC; Simple; p.Gln1367Ter (c.4099C>T); ClinVar=VCV000376060; Zygosity=Homozygous (PubMed=24755471; DepMap).
  • Mutation; HGNC; 2514; CTNNB1; Simple; p.Gly245Ala (c.734G>C); Zygosity=Heterozygous (PubMed=9294210).
  • Mutation; HGNC; 6770; SMAD4; Simple; p.Asp351His (c.1051G>C); ClinVar=VCV000375998; Zygosity=Homozygous (PubMed=24755471; DepMap).
  • Mutation; HGNC; 11998; TP53; Simple; p.Glu204Ter (c.610G>T); ClinVar=VCV000977783; Zygosity=Homozygous (PubMed=16418264; PubMed=24755471; PubMed=28683746; DepMap).
HLA typingSource: PubMed=25960936
Class I
HLA-AA*02:01,02:01
HLA-BB*15:05,56/39
HLA-CC*04:01,04:01

Source: PubMed=26589293
Class I
HLA-AA*02:01,02:01
HLA-BB*15:01,15:01
HLA-CC*04:01,04:01

Source: CLS=300137
Class I
HLA-AA*02:01:01
HLA-BB*15:01:01
HLA-CC*04:01:01
Class II
HLA-DPDPB1*04:01:01
HLA-DQDQA1*03:01:01

DQB1*03:02:01
HLA-DRDRB1*04:04:01
Genome ancestrySource: PubMed=30894373

Origin% genome


African3.33
Native American0
East Asian, North4.59
East Asian, South0
South Asian0
European, North47.35
European, South44.73
DiseaseColon adenocarcinoma (NCIt: C4349)
Species of originHomo sapiens (Human) (NCBI Taxonomy: 9606)
HierarchyChildren:



CVCL_B0YL (Abcam Caco-2 ACE2 KO)CVCL_C0BL (Abcam Caco-2 BMP2 KO)CVCL_C0BM (Abcam Caco-2 TMPRSS2 KO)
CVCL_1096 (C2BBe1)CVCL_Z582 (C2BBe2)CVCL_YP11 (Caco-2 Cu Pro-R)
CVCL_YP12 (Caco-2 CuSO4-R)CVCL_C0R8 (Caco-2 DUSP6 KO)CVCL_C8A0 (Caco-2 EJ6)
CVCL_HA82 (Caco-2-RCAr clone 2)CVCL_B6E1 (Caco-2-SF)CVCL_0232 (Caco-2/15)
CVCL_Z578 (Caco-2/3)CVCL_Z579 (Caco-2/PD-10)CVCL_Z580 (Caco-2/PD-7)
CVCL_Z581 (Caco-2/PF-11)CVCL_0233 (Caco-2/TC-7)
Sex of cellMale
Age at sampling72Y
CategoryCancer cell line
STR profileSource(s): AddexBio; ATCC; CCRID; CLS; DSMZ; ECACC; PubMed=11416159; PubMed=25877200; PubMed=25926053; RCB

Markers:
AmelogeninX
CSF1PO11
D1S165615,16
D2S133817,25
D3S135814,17
D5S81812,13
D7S82011,12 (ATCC; CCRID; CLS; DSMZ; ECACC; PubMed=25877200; PubMed=25926053; RCB)
12 (AddexBio)
D8S117912 (DSMZ; PubMed=11416159)
12,14 (CCRID; CLS; PubMed=25877200)
D10S124813,15
D12S39117,23
D13S31711,13,14
D16S53912,13
D18S5112
D19S43315
D21S1130 (DSMZ; PubMed=11416159; PubMed=25877200)
30,32 (CCRID; CLS; PubMed=25926053)
D22S104515,16
FGA19
Penta D9 (DSMZ)
9,11 (PubMed=25877200)
Penta E7
SE3321
TH016
TPOX9,11
vWA16,18

Run an STR similarity search on this cell line
Web pageshttps://en.wikipedia.org/wiki/Caco-2
https://www.atcc.org/en/support/technical-support/faqs/growth-rate-of-atcc-htb-37
https://www.atcc.org/en/support/technical-support/faqs/morphology-of-atcc-htb-37
https://www.phe-culturecollections.org.uk/media/171256/caco-2-cell-line-profile.pdf
https://www.proteinatlas.org/learn/cellines
http://www.sygnis.com/caco-2/
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/c/cell-lines-detail-37.html
http://www.cyprotex.com/admepk/in-vitro-permeability/caco-2-permeability/
https://www.synapse.org/#!Synapse:syn31544480
https://tcpaportal.org/mclp/
Publications

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=6935474; DOI=10.1093/jnci/66.2.239
Wright W.C., Daniels W.P., Fogh J.
Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.
J. Natl. Cancer Inst. 66:239-247(1981)

PubMed=7459858
Rousset M., Zweibaum A., Fogh J.
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3349466
Chantret I., Barbat A., Dussaulx E., Brattain M.G., Zweibaum A.
Epithelial polarity, villin expression, and enterocytic differentiation of cultured human colon carcinoma cells: a survey of twenty cell lines.
Cancer Res. 48:1936-1942(1988)

PubMed=2914637; DOI=10.1016/0016-5085(89)90897-4
Hidalgo I.J., Raub T.J., Borchardt R.T.
Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability.
Gastroenterology 96:736-749(1989)

PubMed=7764660; DOI=10.1007/BF00749813
Hashimoto K., Shimizu M.
Epithelial properties of human intestinal Caco-2 cells cultured in a serum-free medium.
Cytotechnology 13:175-184(1993)

PubMed=8508948; DOI=10.1111/j.1432-0436.1993.tb00645.x
de Bruine A.P., de Vries J.E., Dinjens W.N.M., Moerkerk P.T.M., van der Linden E.P.M., Pijls M.M.J., ten Kate J., Bosman F.T.
Human Caco-2 cells transfected with c-Ha-Ras as a model for endocrine differentiation in the large intestine.
Differentiation 53:51-60(1993)

PubMed=9294210; DOI=10.1073/pnas.94.19.10330
Ilyas M., Tomlinson I.P.M., Rowan A.J., Pignatelli M., Bodmer W.F.
Beta-catenin mutations in cell lines established from human colorectal cancers.
Proc. Natl. Acad. Sci. U.S.A. 94:10330-10334(1997)

PubMed=10612807; DOI=10.1002/(SICI)1098-2264(200002)27:2<183::aid-gcc10>3.0.CO;2-P
Ghadimi B.M., Sackett D.L., Difilippantonio M.J., Schrock E., Neumann T., Jauho A., Auer G., Ried T.
Centrosome amplification and instability occurs exclusively in aneuploid, but not in diploid colorectal cancer cell lines, and correlates with numerical chromosomal aberrations.
Genes Chromosomes Cancer 27:183-190(2000)

PubMed=10737795; DOI=10.1073/pnas.97.7.3352
Rowan A.J., Lamlum H., Ilyas M., Wheeler J., Straub J., Papadopoulou A., Bicknell D.C., Bodmer W.F., Tomlinson I.P.M.
APC mutations in sporadic colorectal tumors: a mutational 'hotspot' and interdependence of the 'two hits'.
Proc. Natl. Acad. Sci. U.S.A. 97:3352-3357(2000)

PubMed=11414198; DOI=10.1007/s004320000207
Lahm H., Andre S., Hoeflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E., Gabius H.J.
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
J. Cancer Res. Clin. Oncol. 127:375-386(2001)

PubMed=11668190; DOI=10.1177/002215540104901105
Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)

PubMed=12584437; DOI=10.1159/000068544
Melcher R., Koehler S., Steinlein C., Schmid M., Mueller C.R., Luehrs H., Menzel T., Scheppach W., Moerk H., Scheurlen M., Koehrle J., Al-Taie O.
Spectral karyotype analysis of colon cancer cell lines of the tumor suppressor and mutator pathway.
Cytogenet. Genome Res. 98:22-28(2002)

PubMed=14599474; DOI=10.1016/S0887-2333(03)00095-X
Ranaldi G., Consalvo R., Sambuy Y., Scarino M.L.
Permeability characteristics of parental and clonal human intestinal Caco-2 cell lines differentiated in serum-supplemented and serum-free media.
Toxicol. In Vitro 17:761-767(2003)

PubMed=15316659; DOI=10.1007/s00018-004-4222-9
Cinatl J. Jr., Hoever G., Morgenstern B., Preiser W., Vogel J.-U., Hofmann W.-K., Bauer G., Michaelis M., Rabenau H.F., Doerr H.-W.
Infection of cultured intestinal epithelial cells with severe acute respiratory syndrome coronavirus.
Cell. Mol. Life Sci. 61:2100-2112(2004)


Cell line collections (Providers)Abcam; ab275464 - Discontinued
AddexBio; C0009009/29
ATCC; HTB-37
BCRJ; 0059
CCTCC; GDC0153
CLS; 300137
DSMZ; ACC-169
ECACC; 09042001
ECACC; 86010202
IBRC; C10094
ICLC; HTL97023
IZSLER; BS TCL 87
KCB; KCB 200710YJ
KCLB; 30037.1
NCBI_Iran; C139
RCB; RCB0988
Chemistry resourcesChEMBL-Cells; CHEMBL3307519
ChEMBL-Targets; CHEMBL614058
PharmacoDB; Caco2_161_2019
PubChem_Cell_line; CVCL_0025
Encyclopedic resourcesWikidata; Q5016050
Experimental variables resourcesEFO; EFO_0001099

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